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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/8960
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dc.contributor.authorMelidou, M.en
dc.contributor.authorRiganakos, K.en
dc.contributor.authorGalaris, D.en
dc.date.accessioned2015-11-24T16:45:38Z-
dc.date.available2015-11-24T16:45:38Z-
dc.identifier.issn0891-5849-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/8960-
dc.rightsDefault Licence-
dc.subjectcalceinen
dc.subjectcomet assayen
dc.subjectDNA damageen
dc.subjectflavonoidsen
dc.subjecthydrogen peroxideen
dc.subjectiron chelationen
dc.subjectfree radicalsen
dc.subjectantioxidant activity relationshipsen
dc.subjectoxidative damageen
dc.subjectmammalian-cellsen
dc.subjectin-vitroen
dc.subjectpreventionen
dc.subjectquercetinen
dc.subjectapoptosisen
dc.subjectproliferationen
dc.subjectangiogenesisen
dc.subjectfibroblastsen
dc.titleProtection against nuclear DNA damage offered by flavonoids in cells exposed to hydrogen peroxide: The role of iron chelationen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.primaryDOI 10.1016/j.freeradbiomed.2005.08.009-
heal.identifier.secondary<Go to ISI>://000233847700006-
heal.identifier.secondaryhttp://ac.els-cdn.com/S0891584905004405/1-s2.0-S0891584905004405-main.pdf?_tid=3ccb7660a058831261004887fa468264&acdnat=1333037644_3a933744e44191723dad4d58b93f71a9-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.publicationDate2005-
heal.abstractThe ability of a number of flavonoids belonging to the flavone, flavonol, flavanone, and flavan-3-ol subclasses to protect cellular DNA from H2O2-induced single-strand breaks and the underlying molecular mechanisms were investigated in this work. Formation of single-strand breaks on nuclear DNA, after exposure of Jurkat cells to continuously generated H2O2 in the presence or absence of the flavonoid compounds, was evaluated by the comet assay (single-cell gel electrophoresis). The results indicate the following structural requirements of flavonoids for effective DNA protection: (a) the ortho-dihydroxy structure in either ring A or ring B, (b) the hydroxyl moiety on position 3 in combination with the oxo group at position 4, and (c) the presence of a C-2, C-3 double bond in ring C. In contrast to free flavonoids, the ability of complexes of [Fe2+]/[flavonoid] to protect nuclear DNA was decreased as the ratio increased, and the complex was completely inactive when the ratio reached a certain value. Moreover, it was observed that several of the flavonoids tested were able to remove iron from calcein loaded into cells and that this property was in excellent correlation with their ability to protect DNA (Speartnan's correlation coefficient, p = 0.9, p = 0.005). The antioxidant (electron donating) capacities of the same flavonoids were also evaluated by a conventional method, but no relation with their DNA-protective ability could be established even when their membrane-penetrating abilities were taken into account (p = 0.64). In conclusion, the results presented in this work strongly support the notion that intracellular binding of iron is responsible for the protection offered by flavonoids against H2O2-induced DNA damage. (c) 2005 Elsevier Inc. All rights reserved.en
heal.publisherElsevieren
heal.journalNameFree Radical Biology and Medicineen
heal.journalTypepeer reviewed-
heal.fullTextAvailabilityTRUE-
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