Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/8425
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dc.contributor.authorYao, D. H.en
dc.contributor.authorProdromidis, M. I.en
dc.contributor.authorVlessidis, A. G.en
dc.contributor.authorKarayannis, M. I.en
dc.contributor.authorEvmiridis, N. P.en
dc.date.accessioned2015-11-24T16:41:28Z-
dc.date.available2015-11-24T16:41:28Z-
dc.identifier.issn0003-2670-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/8425-
dc.rightsDefault Licence-
dc.subjectnitric oxideen
dc.subjectnafion-cellulose acetate composite membraneen
dc.subjectbiological fluid samplingen
dc.subjectchemiluminescenceen
dc.subjectnitric-oxideen
dc.subjectonline detectionen
dc.subjectl-arginineen
dc.subjectelectrodesen
dc.subjectselectivityen
dc.subjectmechanismen
dc.subjectmanifolden
dc.subjectsensoren
dc.subjectphaseen
dc.subjectfilmsen
dc.titleMembrane sampler for interference-free flow injection NO determination in biological fluids with chemiluminescence detectionen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.primaryDoi 10.1016/S0003-2670(01)01369-1-
heal.identifier.secondary<Go to ISI>://000172657500007-
heal.identifier.secondaryhttp://ac.els-cdn.com/S0003267001013691/1-s2.0-S0003267001013691-main.pdf?_tid=8f62f70a-356a-11e3-aafb-00000aacb35f&acdnat=1381821934_4788dd16eb3d44ff60f729a8beeb5d32-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.publicationDate2001-
heal.abstractThe development of a chemiluminescence (CL) method based on the perm-selective properties of a Nafion-cellulose acetate (CA) composite membrane for the monitoring of nitric oxide (NO) in biological fluids is described. Horseradish peroxidase (HRP) was used as NO trapping solution, forming the stable compound HRP-NO. The HRP was denatured and the trapped NO was released and detected by using the luminol-H2O2 system. Using a mixed (size-exclusion and polar-based) transport control, the interference effects of various compounds were minimized. The method was used for NO monitoring in simulated samples, by using a blood specimen as sample matrix. The 3 sigma detection limit is 0.9 x 10(-6) mol and linear semi-log calibration plot in the range 1.8 x 10(-6) to 2.7 x 10(-3) mol NO was constructed. The applied methodology was further used to prolong the NO lifetime in order to increase the sensitivity of its determination. This was based on the increase of the response in the presence of certain reductive species, which act as NO preservatives in biological fluid samples. (C) 2001 Elsevier Science B.V All rights reserved.en
heal.publisherElsevier Massonen
heal.journalNameAnalytica Chimica Actaen
heal.journalTypepeer reviewed-
heal.fullTextAvailabilityTRUE-
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά). ΧΗΜ

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