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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Douka, E. | en |
| dc.contributor.author | Koukkou, A. I. | en |
| dc.contributor.author | Vartholomatos, G. | en |
| dc.contributor.author | Frillingos, S. | en |
| dc.contributor.author | Papamichael, E. M. | en |
| dc.contributor.author | Drainas, C. | en |
| dc.date.accessioned | 2015-11-24T16:40:55Z | - |
| dc.date.available | 2015-11-24T16:40:55Z | - |
| dc.identifier.issn | 0021-9193 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/8333 | - |
| dc.rights | Default Licence | - |
| dc.subject | escherichia-coli | en |
| dc.subject | pseudomonas-aeruginosa | en |
| dc.subject | reca gene | en |
| dc.subject | transport | en |
| dc.subject | expression | en |
| dc.subject | ethanol | en |
| dc.subject | cloning | en |
| dc.subject | operon | en |
| dc.subject | metabolism | en |
| dc.subject | sequence | en |
| dc.title | A Zymomonas mobilis mutant with delayed growth on high glucose concentrations | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.secondary | <Go to ISI>://000081706100022 | - |
| heal.identifier.secondary | http://jb.asm.org/content/181/15/4598.full.pdf | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας | el |
| heal.publicationDate | 1999 | - |
| heal.abstract | Exponentially growing cells of Zymomonas mobilis normally exhibit a lag period of up to 3 h when transferred from 0.11 M (2%) to 0.55 M (10%) glucose liquid medium. A mutant of Z, mobilis (CU1Rif2), fortuitously isolated, showed more than a 20-h lag period when grown under the same conditions, whereas on 0.55 M glucose solid medium, it failed to grow. The growth of CU1Rif2 on elevated concentrations of other fermentable (0.55 M sucrose or fructose) or nonfermentable (0.11 M glucose plus 0.44 M maltose or xylose) sugars appeared to be normal, Surprisingly, CU1Rif2 cells grew without any delay on 0.55 M glucose on which wild-type cells had been incubated for 3 h and removed at the beginning of their exponential phase. This apparent preconditioning was not observed with medium obtained from wild-type cells grown on 0.11 M glucose and supplemented to 0.55 M after removal of the wild-type cells. Undelayed growth of CU1Rif2 on 0.55 M glucose previously conditioned by the wild type was impaired by heating or protease treatment. It is suggested that in Z, mobilis, a diffusible proteinaceous heat-labile factor, transitionally not present in 0.55 M glucose CU1Rif2 cultures, triggers growth on 0.55 M glucose. Biochemical analysis of glucose uptake and glycolytic enzymes implied that glucose assimilation was not directly involved in the phenomenon. By use of a wild-type Z. mobilis genomic library, a 4.5-kb DNA fragment which complemented in low copy number the glucose-defective phenotype as well as glucokinase and glucose uptake of CU1Rif2 was isolated. This fragment carries a gene cluster consisting of Four putative coding regions, encoding 167, 167, 145, and 220 amino acids with typical Z, mobilis codon usage, -35 and -10 promoter elements, and individual Shine-Dalgarno consensus sites. However, strong homologies were not deterred in a BLAST2 (EMBL-Heidelberg) computer search with known protein sequences. | en |
| heal.publisher | American Society for Microbiology | en |
| heal.journalName | J Bacteriol | en |
| heal.journalType | peer reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά). ΧΗΜ | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Papamichael-1999-A Zymomonas mobilis.pdf | 183.19 kB | Adobe PDF | View/Open |
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