Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/7998
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dc.contributor.authorStalikas, C. D.en
dc.contributor.authorKarayannis, M. I.en
dc.contributor.authorTzouwarakarayanni, S. M.en
dc.date.accessioned2015-11-24T16:38:05Z-
dc.date.available2015-11-24T16:38:05Z-
dc.identifier.issn0939-4974-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/7998-
dc.rightsDefault Licence-
dc.subjectamino-acid-analysisen
dc.subjectchromatographyen
dc.subjectderivatizationen
dc.subjectsystemen
dc.titleFlow-Injection Determination of Glutamate in Human Serum and Rat-Brain Samples with Immobilized Glutamate Oxidase and Glutamate-Dehydrogenase Reactorsen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.secondary<Go to ISI>://A1994PP67800007-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.publicationDate1994-
heal.abstractGlutamate oxidase was immobilized on non-porous glass beads and glutamate dehydrogenase was immobilized on glass derivatives. These supports were employed for the construction of Single Bead String Reactors and Packed Bed Reactors, respectively, which in turn were linked to Flow Injection Analysis systems with either photometric or fluorometric detection. Analytical working curves are linear in the range 1-200 mu mol/l for packed bed reactors and 10-500 mmol/l for single bead string reactors. The samples were pretreated depending on their origin and the applied measuring system. Optimal dilution factors were established for the two techniques. Optimal dilution ratios were established and the influence of several added substances was investigated. Recovery and method comparison studies including high performance liquid chromatography verified the accuracy of the proposed methods. Results from within-day and between-day measurements gave relative standard deviations of 4.7 and 5.9% for serum samples and 2.5 and 4.0% for brain samples, respectively.en
heal.publisherWalter de Gruyter & Co.en
heal.journalNameEuropean Journal of Clinical Chemistry and Clinical Biochemistryen
heal.journalTypepeer reviewed-
heal.fullTextAvailabilityTRUE-
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά). ΧΗΜ

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