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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Liopeta, K. | en |
| dc.contributor.author | Boubali, S. | en |
| dc.contributor.author | Virgilio, L. | en |
| dc.contributor.author | Thyphronitis, G. | en |
| dc.contributor.author | Mavrothalassitis, G. | en |
| dc.contributor.author | Dimitracopoulos, G. | en |
| dc.contributor.author | Paliogianni, F. | en |
| dc.date.accessioned | 2015-11-24T16:34:14Z | - |
| dc.date.available | 2015-11-24T16:34:14Z | - |
| dc.identifier.issn | 0161-5890 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/7779 | - |
| dc.rights | Default Licence | - |
| dc.subject | camp | en |
| dc.subject | il-10 | en |
| dc.subject | t lymphocytes | en |
| dc.subject | mef2 | en |
| dc.subject | pka | en |
| dc.subject | myocyte-enhancer factor-2 | en |
| dc.subject | protein-kinase-a | en |
| dc.subject | necrosis-factor-alpha | en |
| dc.subject | gene-expression | en |
| dc.subject | transcription factors | en |
| dc.subject | interleukin-10 promoter | en |
| dc.subject | cell-proliferation | en |
| dc.subject | muscle development | en |
| dc.subject | prostaglandin e-2 | en |
| dc.subject | binding protein | en |
| dc.title | cAMP regulates IL-10 production by normal human T lymphocytes at multiple levels: A potential role for MEF2 | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.primary | DOI 10.1016/j.molimm.2008.10.025 | - |
| heal.identifier.secondary | <Go to ISI>://000262810400005 | - |
| heal.identifier.secondary | http://ac.els-cdn.com/S0161589008007438/1-s2.0-S0161589008007438-main.pdf?_tid=44e2cca8468d07c16b2bdd726b6f207a&acdnat=1336730790_1fe974404445ea4d356893933d908dab | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών | el |
| heal.publicationDate | 2009 | - |
| heal.abstract | Signal transduction by the cAMP/cAMP-dependent protein kinase A (PKA) pathway is triggered through multiple receptors and is important for many processes in a variety of cells. In T cells, the engagement of the TCR-CD3 complex induces CAMP, a second messenger that controls immune response. IL-10, produced by a variety of lymphocyte subpopulations, is an important regulator of this response exerting a wide range of immunomodulatory actions. Elevation of cAMP has been shown to increase IL-10 production by monocytes. However, the mechanism of cAMP mediated regulation of IL-10 production by T lymphocytes remains unclear. In this study using normal peripheral T lymphocytes stimulated either through the TCR-CD3 complex or the TCR-CD3 and the CD28 molecule, we show that IL-10 is produced mainly by memory T lymphocytes after either way of stimulation and is drastically inhibited (70-90%) by CAMP elevating agents. cAMP mediated inhibition was reversed by the use of the specific PKA inhibitor Rp-8-Br-cAMP but not by the addition of exogenous rhIL-2, indicating that the inhibitory effect depends on PKA activation and is not secondary to IL-2 inhibition, Inhibition is taking place at both transcriptional and posttranscriptional level. Transfection of a luciferase reporter plasmid carrying the IL-10 promoter in T cells, revealed that TCR/CD28-induced activation was inhibited by 60% by cAMP elevation. The most sensitive part to cAMP mediated inhibition was a fragment of 135 bp Upstream of TATA box, which contains multiple binding sites for MEF-2. Overexpression of MEF-2 in the same cells increased IL-10 promoter activity by 2.5-fold. Stimulation through TCR/CD28 increased MEF-2 binding in its Corresponding binding sites which was inhibited by 80% in the presence of cAMP elevating agents. These results suggest that the inhibitory effect of cAMP on IL-10 production by normal peripheral T lymphocytes is cell type and stimulus specific, exerted on multiple levels and involves MEF2 transcription factor. (C) 2008 Elsevier Ltd. All rights reserved. | en |
| heal.journalName | Mol Immunol | en |
| heal.journalType | peer reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Liopeta-2009-cAMP regulates IL-10.pdf | 890.59 kB | Adobe PDF | View/Open Request a copy |
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