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  5. Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)
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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/7654
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dc.contributor.authorMarangos, P.en
dc.contributor.authorCarroll, J.en
dc.date.accessioned2015-11-24T16:33:24Z-
dc.date.available2015-11-24T16:33:24Z-
dc.identifier.issn1470-1626-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/7654-
dc.rightsDefault Licence-
dc.subjectAnimalsen
dc.subjectBiological Transporten
dc.subjectCell Nucleus/metabolismen
dc.subjectCells, Cultureden
dc.subjectCyclin B/*analysis/geneticsen
dc.subjectCyclin B1en
dc.subjectCytoplasm/metabolismen
dc.subjectFemaleen
dc.subjectMaturation-Promoting Factor/analysisen
dc.subjectMeiosis/*physiologyen
dc.subjectMiceen
dc.subjectMice, Inbred Strainsen
dc.subjectMicroscopy, Confocalen
dc.subjectMen
dc.titleThe dynamics of cyclin B1 distribution during meiosis I in mouse oocytesen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.primary10.1530/rep.1.00192-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/15280554-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιώνel
heal.publicationDate2004-
heal.abstractCdk1-cyclin B1 kinase activity drives oocytes through meiotic maturation. It is regulated by the phosphorylation status of cdk1 and by its spatial organisation. Here we used a cyclin B1-green fluorescent protein (GFP) fusion protein to examine the dynamics of cdk1-cyclin B1 distribution during meiosis I (MI) in living mouse oocytes. Microinjection of cyclin B1-GFP accelerated germinal vesicle breakdown (GVBD) and, as previously described, overrides cAMP-mediated meiotic arrest. GVBD was pre-empted by a translocation of cyclin B1-GFP from the cytoplasm to the germinal vesicle (GV). After nuclear accumulation, cyclin B1-GFP localised to the chromatin. The localisation of cyclin B1-GFP is governed by nuclear import and export. In GV intact oocytes, cyclin export was demonstrated by showing that cyclin B1-GFP injected into the GV is exported to the cytoplasm while a similar size dextran is retained. Import was revealed by the finding that cyclin B1-GFP accumulated in the GV when export was inhibited using leptomycin B. These studies show that GVBD in mouse oocytes is sensitive to cyclin B1 abundance and that the changes in distribution of cyclin B1 contribute to progression through MI.en
heal.journalNameReproductionen
heal.journalTypepeer reviewed-
heal.fullTextAvailabilityTRUE-
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