Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/7645
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dc.contributor.authorKatapodis, P.en
dc.contributor.authorKalogeris, E.en
dc.contributor.authorKekos, D.en
dc.contributor.authorMacris, B. J.en
dc.contributor.authorChristakopoulos, P.en
dc.date.accessioned2015-11-24T16:33:19Z-
dc.date.available2015-11-24T16:33:19Z-
dc.identifier.issn0175-7598-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/7645-
dc.rightsDefault Licence-
dc.subjectAmino Acids/analysis/chemistryen
dc.subjectCarbohydrates/analysis/chemistryen
dc.subjectCell Membrane/chemistryen
dc.subjectChromatography, High Pressure Liquiden
dc.subjectCulture Media/chemistryen
dc.subjectCytoplasm/chemistryen
dc.subjectHydrogen-Ion Concentrationen
dc.subjectMembrane Fluidityen
dc.subjectMembrane Lipids/analysisen
dc.subjectOligoen
dc.titleBiosynthesis of fructo-oligosaccharides by Sporotrichum thermophile during submerged batch cultivation in high sucrose mediaen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.primary10.1007/s00253-003-1348-x-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/12802527-
heal.identifier.secondaryhttp://link.springer.com/content/pdf/10.1007%2Fs00253-003-1348-x.pdf-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιώνel
heal.publicationDate2004-
heal.abstractBiosynthesis of fructo-oligosaccharides (FOS) was observed during growth of the thermophilic fungus Sporotrichum thermophile on media containing high sucrose concentrations. Submerged batch cultivation with the optimum initial sucrose concentration of 250 g/l allowed the production of 12.5 g FOS/l. The FOS mixture obtained was composed of three sugars, which were isolated by size-exclusion chromatography. They were characterized by acid hydrolysis and HPLC as 1-kestose, 6-kestose and neokestose. The mechanism of osmotic adaptation of S. thermophile was investigated and sugars and amino acids were found to be the predominant compatible solutes. The fungus accumulated glutamic acid, arginine, alanine, leucine and lysine, in order to balance the outer osmotic pressure. Fatty acid analysis of the membrane lipids showed a relatively high percentage of unsaturated lipids, which is known to be associated with high membrane fluidity.en
heal.journalNameAppl Microbiol Biotechnolen
heal.journalTypepeer reviewed-
heal.fullTextAvailabilityTRUE-
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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