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dc.contributor.authorFranqueville, E.en
dc.contributor.authorStamatis, H.en
dc.contributor.authorLoutrari, H.en
dc.contributor.authorFriboulet, A.en
dc.contributor.authorKolisis, F.en
dc.rightsDefault Licence-
dc.subjectcatalytic antibodyen
dc.subjectreverse micelleen
dc.subjectaot surfactanten
dc.subjectreverse micellesen
dc.subjectbioorganic reactionsen
dc.subjectantibody catalysisen
dc.titleStudies on the catalytic behaviour of a cholinesterase-like abzyme in an AOT microemulsion systemen
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.secondary<Go to ISI>://000177080000007-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιώνel
heal.abstractThe hydrolytic activity of a monoclonal catalytic antibody (9A8) (abzyme) with acetylcholinesterase-like activity was investigated in water-in-oil (w/o) microemulsions (reverse micelles) based on sodium his-2-(ethylhexyl)sulfosuccinate (AOT) in isooctane, using p- and o-nitrophenylacetate (p-and o-NPA) as substrates. The dependence of the abzyme hydrolytic activity on the molar ratio of water to surfactant (w(o)) showed a bell-shaped curve, presenting a maximum at w(o) = 11.1. An increase of the AOT concentration at constant w(o), resulted in a decrease of the catalytic activity suggesting a possible inhibition effect of the surfactant. The incorporation of the abzyme into the reverse micelle system caused a blue shift of the fluorescence emission maximum by a magnitude of 7-10 nm depending on the w(o) value. This result indicates that the antibody molecule, or a large part of it, is located in the aqueous tnicrophase of the system. Kinetic studies showed that the hydrolysis of p-and o-NPA in microemulsion system as well as in aqueous solution follows Michaelis-Menten kinetics. The catalytic efficiency (k(cat)/K-m) in w/o microemulsion was significant lower than in aqueous solution. (C) 2002 Elsevier Science B.V. All rights reserved.en
heal.journalNameJournal of Biotechnologyen
heal.journalTypepeer reviewed-
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