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  5. Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)
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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/7495
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dc.contributor.authorPerez, S.en
dc.contributor.authorCourtis, N.en
dc.contributor.authorKokkinopoulos, D.en
dc.contributor.authorPapamichail, M.en
dc.contributor.authorTsiapalis, C. M.en
dc.contributor.authorTrangas, T.en
dc.date.accessioned2015-11-24T16:31:54Z-
dc.date.available2015-11-24T16:31:54Z-
dc.identifier.issn0022-1759-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/7495-
dc.rightsDefault Licence-
dc.subject5'-Nucleotidaseen
dc.subjectAdenosine Deaminase/pharmacologyen
dc.subjectAnimalsen
dc.subjectBiological Assayen
dc.subjectCell Lineen
dc.subjectColorimetry/*methodsen
dc.subjectHumansen
dc.subjectLeukemia, Experimental/enzymologyen
dc.subjectLeukemia, Myeloid/enzymologyen
dc.subjectLeukocytes/enzymologyen
dc.subjectMiceen
dc.subjectMicrochemistryen
dc.subjectNucleotidases/*blooden
dc.subjectRaen
dc.titleA colorimetric assay for the determination of 5'-nucleotidase activityen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/3039004-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιώνel
heal.publicationDate1987-
heal.abstractA rapid, simple, quantitative and sensitive assay for the determination of 5'-nucleotidase has been developed. The method can be applied to both soluble and membrane bound forms of the leukocyte enzyme. Enzyme activity is determined by colorimetric estimation of NH3 released from adenosine, the product of 5'-nucleotidase activity in the presence of adenosine deaminase. The assay may be performed in microtitre plates and read with an automatic multiscan spectrophotometer. Thus it can be applied to a large number of samples for routine medical and research purposes.en
heal.journalNameJ Immunol Methodsen
heal.journalTypepeer reviewed-
heal.fullTextAvailabilityTRUE-
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