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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Paliogianni, F. | en |
| dc.contributor.author | Hama, N. | en |
| dc.contributor.author | Mavrothalassitis, G. J. | en |
| dc.contributor.author | Thyphronitis, G. | en |
| dc.contributor.author | Boumpas, D. T. | en |
| dc.date.accessioned | 2015-11-24T16:31:34Z | - |
| dc.date.available | 2015-11-24T16:31:34Z | - |
| dc.identifier.issn | 0008-8749 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/7460 | - |
| dc.rights | Default Licence | - |
| dc.subject | chromosomal gene | en |
| dc.subject | phorbol ester | en |
| dc.subject | lymphocytes-t | en |
| dc.subject | calcineurin | en |
| dc.subject | transcription | en |
| dc.subject | il-4 | en |
| dc.subject | camp | en |
| dc.subject | prostaglandin-e2 | en |
| dc.subject | cyclosporine | en |
| dc.subject | t-helper-1 | en |
| dc.title | Signal requirements for interleukin 4 promoter activation in human T cells | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.secondary | <Go to ISI>://A1996TY85400004 | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών | el |
| heal.publicationDate | 1996 | - |
| heal.abstract | We have studied the signal requirements for human IL-4 promoter activation in Jurkat T cells by the use of DNA transfection assays with vectors carrying the IL-4 promoter linked to a reporter gene, Stimulation with calcium (Ca2+) ionophores (ionomycin), but not with phorbol esters (phorbol myristate acetate, PMA) or cyclic AMP elevating agents (prostaglandin Ea(2) PGE(2)), induced the transcriptional activity of the IL-4 promoter by similar to 3-fold. Costimulation with ionomycin and PGE(2) resulted in the same level of IL-4 promoter activity as the stimulation with ionomycin alone. In contrast, costimulation with ionomycin and PMA decreased the activity of the IL-4 promoter by similar to 40% compared to stimulation with ionomycin alone. Induction of IL-4 promoter by ionomycin was partially inhibited (similar to 50% inhibition) in the presence of as high as 2 mu g/ml cyclosporin A (CsA), an inhibitor of the Ca+/calmodulin-dependent phosphatase calcineurin. Under the same conditions, only 0.1 mu g/ml of CsA inhibited by >95% the transactivation of the IL-2 promoter in response to ionomycin and PMA. Transfection of a deletion mutant of the calcineurin catalytic subunit (Delta CaM-AI) known to have Ca2+-independent, constitutive phosphatase activity increased IL-4 promoter activity by similar to 14-fold. Stimulation with ionomycin of cells transfected with low doses of Delta CaM-AI, further induced IL-4 promoter activity by similar to 2-fold, These results identify the Ca2+-signaling system as a key component of the signal transduction pathway leading to IL-4 promoter activation in Jurkat T cells and suggest a major role of calcineurin in its regulation. (C) 1996 Academic Press, Inc. | en |
| heal.journalName | Cell Immunol | en |
| heal.journalType | peer reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) | |
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