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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/24628
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dc.contributor.authorHayajneh, W. A.en
dc.contributor.authorContopoulos-Ioannidis, D. G.en
dc.contributor.authorLesperance, M. M.en
dc.contributor.authorVenegas, A. M.en
dc.contributor.authorColberg-Poley, A. M.en
dc.date.accessioned2015-11-24T19:42:21Z-
dc.date.available2015-11-24T19:42:21Z-
dc.identifier.issn0022-1317-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/24628-
dc.rightsDefault Licence-
dc.subjectAmino Acid Sequenceen
dc.subjectCell Lineen
dc.subjectChloramphenicol O-Acetyltransferaseen
dc.subjectCytomegalovirus/*genetics/metabolismen
dc.subjectEndoplasmic Reticulum/metabolismen
dc.subjectGenetic Variationen
dc.subjectGlycoproteins/genetics/metabolismen
dc.subjectHSP70 Heat-Shock Proteins/geneticsen
dc.subjectHumansen
dc.subjectImmediate-Early Proteins/*genetics/metabolismen
dc.subjectMitochondria/metabolismen
dc.subjectMolecular Sequence Dataen
dc.subjectMutationen
dc.subjectOpen Reading Framesen
dc.subjectPhylogenyen
dc.subjectPromoter Regions, Geneticen
dc.subject*Transcriptional Activationen
dc.subjectTransfectionen
dc.subjectViral Proteins/*genetics/metabolismen
dc.titleThe carboxyl terminus of the human cytomegalovirus UL37 immediate-early glycoprotein is conserved in primary strains and is important for transactivationen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/11413367-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate2001-
heal.abstractThe human cytomegalovirus (HCMV) UL37 exon 3 (UL37x3) open reading frame (ORF) encodes the carboxyl termini of two immediate-early glycoproteins (gpUL37 and gpUL37(M)). UL37x3 homologous sequences are not required for mouse cytomegalovirus (MCMV) growth in vitro; yet, they are important for MCMV growth and pathogenesis in vivo. Similarly, UL37x3 sequences are dispensable for HCMV growth in culture, but their requirement for HCMV growth in vivo is not known. To determine this requirement, we directly sequenced the complete UL37x3 gene in multiple HCMV primary strains. A total of 63 of the 310 amino acids in the UL37x3 ORF differ non-conservatively in one or more HCMV primary strains. The HCMV UL37x3 genetic diversity is non-random: the N-glycosylation (46/186 aa) and basic (9/15 aa) domains have the highest proportion of non-conservative variant amino acids. Nonetheless, most (15/17 signals) of the N-glycosylation signals are retained in all HCMV primary strains. Moreover, new N-glycosylation signals are encoded by 5/20 primary strains. In sharp contrast, the UL37x3 transmembrane (TM) ORF completely lacks diversity in all 20 HCMV sequenced primary strains, and only 1 of 28 cytosolic tail residues differs non-conservatively. To test the functional significance of the conserved carboxyl terminus, gpUL37 mutants lacking the TM and/or cytosolic tail were tested for transactivating activity. The gpUL37 carboxyl-terminal mutants are partially defective in hsp70 promoter transactivation even though they trafficked similarly to the wild-type protein into the endoplasmic reticulum and to mitochondria. From these results, we conclude that N-glycosylated gpUL37, particularly its TM and cytosolic domains, is important for HCMV growth in humans.en
heal.journalNameJ Gen Virolen
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
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