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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Grozdanovic, Z. | en |
| dc.contributor.author | Nakos, G. | en |
| dc.contributor.author | Mayer, B. | en |
| dc.contributor.author | Gossrau, R. | en |
| dc.date.accessioned | 2015-11-24T19:40:14Z | - |
| dc.date.available | 2015-11-24T19:40:14Z | - |
| dc.identifier.issn | 0239-8508 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/24319 | - |
| dc.rights | Default Licence | - |
| dc.subject | Animals | en |
| dc.subject | Female | en |
| dc.subject | Fluorescent Antibody Technique | en |
| dc.subject | Formaldehyde | en |
| dc.subject | Guinea Pigs | en |
| dc.subject | Histocytochemistry/*methods | en |
| dc.subject | Histocytological Preparation Techniques | en |
| dc.subject | Immunohistochemistry/methods | en |
| dc.subject | Male | en |
| dc.subject | Mice | en |
| dc.subject | NADPH Dehydrogenase/*metabolism | en |
| dc.subject | Neurons/enzymology | en |
| dc.subject | Nitric Oxide/*biosynthesis | en |
| dc.subject | Nitric Oxide Synthase/*analysis | en |
| dc.subject | Rats | en |
| dc.subject | Rats, Wistar | en |
| dc.subject | Substrate Specificity | en |
| dc.title | A modified method allows for correlation between NADPH-diaphorase histochemistry and immunohistochemistry for the demonstration of neuronal nitric oxide synthase (nNOS) | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/7556767 | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.publicationDate | 1995 | - |
| heal.abstract | Results obtained with the conventional nitro blue tetrazolium salt method for the visualization of the NADPH-diaphorase (NADPH-d) activity of nitric oxide synthase (NOS) are not specific for this particular enzyme, since this activity represents only a fraction of the total cellular NADPH-d pool. Therefore, the standard NADPH-d procedure was modified by performing the incubation in the presence of formaldehyde. Parallel application of the modified NADPH-d staining technique and the indirect immunofluorescence using an antibody against the neuronal isoenzyme (nNOS) on rat, mouse and guinea-pig tissues showed a correlation between histochemical and immunocytochemical staining. It can thus be concluded that the modified NADPH-d procedure allows for a more specific detection of the histochemical nNOS activity than the conventional method. | en |
| heal.journalName | Folia Histochem Cytobiol | en |
| heal.journalType | peer-reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ | |
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