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dc.contributor.authorDalekos, G. N.en
dc.contributor.authorBoumba, D. S.en
dc.contributor.authorKatopodis, K.en
dc.contributor.authorZervou, E.en
dc.contributor.authorSferopoulos, G.en
dc.contributor.authorElisaf, M. S.en
dc.contributor.authorTsianos, E. V.en
dc.contributor.authorSiamopoulos, K. C.en
dc.rightsDefault Licence-
dc.subjectHepacivirus/genetics/*isolation & purificationen
dc.subjectHepatitis Antibodies/*blooden
dc.subjectHepatitis C/*diagnosis/immunology/virologyen
dc.subjectImmunoenzyme Techniquesen
dc.subjectMiddle Ageden
dc.subjectPolymerase Chain Reactionen
dc.subjectRNA, Viral/blood/geneticsen
dc.subjectRenal Dialysis/*adverse effectsen
dc.titleAbsence of HCV viraemia in anti-HCV-negative haemodialysis patientsen
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.abstractBACKGROUND: Immunologic alterations have been reported in chronic haemodialysis (HD) patients. Some HD patients may have, therefore, an inability to produce detectable amounts of serum antibodies to hepatitis C virus (anti-HCV). Previous studies have shown the presence of HCV viraemia in anti-HCV-negative HD patients (ranging from 1 to 15%). However, the universal epidemiologic impact of these cases remains uncertain since there are conflicting results. In this context, we conducted a study in an attempt to investigate the presence of HCV viraemia among anti-HCV-negative HD patients in a well-defined geographic area of the northwestern part of Greece. METHODS: During a 6 month period, 81 anti-HCV-negative HD patients were tested twice for the presence of HCV RNA, using the reverse transcriptase polymerase chain reaction (RT-PCR) combined with a DNA enzyme immunoassay (DEIA). At the same time, periodic testing for anti-HCV by two commercially available third generation assays was done. In addition, 15 anti-HCV-positive HD patients and 20 non-HD patients with well established chronic HCV infection used as internal controls were tested for the presence of HCV RNA and anti-HCV. RESULTS: None of the anti-HCV-negative HD patients were shown to be viraemic by the combined RT-PCR and DEIA method. During the same time period, all remained anti-HCV negative by the third generation assays. By contrast, all the patients with known HCV-infection were positive by the two enzyme immunoassays, whereas 13 anti-HCV-positive HD patients (86.7%) and 18 non-HD patients (90%) were viraemic by RT-PCR. CONCLUSIONS: This study demonstrated that routine HCV RNA testing in anti-HCV-negative HD patients appears not to be necessary particularly when third generation assays are used for the detection of anti-HCV.en
heal.journalNameNephrol Dial Transplanten
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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