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https://olympias.lib.uoi.gr/jspui/handle/123456789/24118Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Sahin-Toth, M. | en |
| dc.contributor.author | Frillingos, S. | en |
| dc.contributor.author | Lengeler, J. W. | en |
| dc.contributor.author | Kaback, H. R. | en |
| dc.date.accessioned | 2015-11-24T19:38:10Z | - |
| dc.date.available | 2015-11-24T19:38:10Z | - |
| dc.identifier.issn | 0006-291X | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/24118 | - |
| dc.rights | Default Licence | - |
| dc.subject | Base Sequence | en |
| dc.subject | Biological Transport, Active | en |
| dc.subject | Cell Membrane/enzymology | en |
| dc.subject | Cloning, Molecular | en |
| dc.subject | DNA, Bacterial/chemistry/metabolism | en |
| dc.subject | Epitopes/analysis | en |
| dc.subject | Escherichia coli/*enzymology/genetics | en |
| dc.subject | Escherichia coli Proteins | en |
| dc.subject | Gene Expression | en |
| dc.subject | *Genes, Bacterial | en |
| dc.subject | Kinetics | en |
| dc.subject | Membrane Transport Proteins/biosynthesis/*metabolism | en |
| dc.subject | Molecular Sequence Data | en |
| dc.subject | Plasmids | en |
| dc.subject | Promoter Regions, Genetic | en |
| dc.subject | Protein Biosynthesis | en |
| dc.subject | Sucrose/metabolism | en |
| dc.subject | beta-Galactosidase/genetics | en |
| dc.title | Active transport by the CscB permease in Escherichia coli K-12 | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/7535526 | - |
| heal.identifier.secondary | http://ac.els-cdn.com/S0006291X85714490/1-s2.0-S0006291X85714490-main.pdf?_tid=1671da125de5526681f1237c87b72359&acdnat=1332914788_1cd53f0af1aa67a75405945215a16618 | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.publicationDate | 1995 | - |
| heal.abstract | The cscB gene encoding a putative sucrose permease from Escherichia coli EC3132 [Bockmann, J., Heuel, H. & Lengeler, J.W. (1992) Mol. Gen. Genet. 235, 22-32] was placed under control of the lacZ promoter/operator and overexpressed. Cells of E. coli K-12 expressing cscB gene product from a high copy number plasmid accumulate sucrose against a concentration gradient with an apparent Km of about 1 mM and a Vmax of approximately 23 nmol/min/mg protein. In contrast, transport of lactose or melibiose is not observed. Epitope-tagging and immunological analysis reveal that concomitant with increased transport activity, a protein with an apparent M(r) of 33-34 kDa appears in the cytoplasmic membrane. The results provide direct support for the contention that the cscB gene encodes a sucrose permease and provide the basis for further molecular analysis. | en |
| heal.journalName | Biochem Biophys Res Commun | en |
| heal.journalType | peer-reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Sahin-Toth-1995-Active transport by.pdf | 419.86 kB | Adobe PDF | View/Open Request a copy |
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