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https://olympias.lib.uoi.gr/jspui/handle/123456789/23952Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Otero, G. | en |
| dc.contributor.author | Avila, M. A. | en |
| dc.contributor.author | de la Pena, L. | en |
| dc.contributor.author | Emfietzoglou, D. | en |
| dc.contributor.author | Cansado, J. | en |
| dc.contributor.author | Popescu, G. F. | en |
| dc.contributor.author | Notario, V. | en |
| dc.date.accessioned | 2015-11-24T19:36:51Z | - |
| dc.date.available | 2015-11-24T19:36:51Z | - |
| dc.identifier.issn | 0143-3334 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/23952 | - |
| dc.rights | Default Licence | - |
| dc.subject | Animals | en |
| dc.subject | *Cell Line, Transformed | en |
| dc.subject | Cell Transplantation | en |
| dc.subject | Cells, Cultured | en |
| dc.subject | Cloning, Molecular | en |
| dc.subject | Cricetinae | en |
| dc.subject | Electron Transport Complex IV/biosynthesis/*genetics/radiation effects | en |
| dc.subject | Gene Expression Regulation/radiation effects | en |
| dc.subject | Hydrogen Peroxide/metabolism | en |
| dc.subject | Mice | en |
| dc.subject | Mice, Nude | en |
| dc.subject | Protein Precursors/genetics | en |
| dc.subject | RNA Processing, Post-Transcriptional/*radiation effects | en |
| dc.subject | RNA, Messenger/analysis | en |
| dc.title | Altered processing of precursor transcripts and increased levels of the subunit I of mitochondrial cytochrome c oxidase in Syrian hamster fetal cells initiated with ionizing radiation | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/9276632 | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.publicationDate | 1997 | - |
| heal.abstract | Treatment of Syrian hamster fetal cells (SHFC) with ionizing radiation resulted in the establishment of 21 transformed cell lines. Relative to unirradiated controls, cells from early post-irradiation passages (p.3) showed marked morphologic alterations, increased growth rate and extended life span, and they were contact-inhibited and not tumorigenic in nude mice, although they became tumorigenic after extended passaging in culture (p. > 30). Differential mRNA display analyses of normal cells (84-3) and radiation-initiated cell lines at early passage showed that the latter contained increased steady-state levels of the precursor (4-fold) and mature (1.7-fold) transcripts of the mitochondrial (mt) gene encoding the subunit I of cytochrome c oxidase (CO I). These molecular alterations were consistently observed in 57% of the irradiated (HDR) cell lines, and were stably maintained during continuous passaging (p. > 50). Further analyses of one of these cell lines (HDR-3) demonstrated that the accumulation of CO I precursor transcripts was the result of mRNA stabilization and increased replication and/or amplification of the mt DNA. Radiation-initiated cells contained elevated levels of the CO I protein, showed a 75% reduction in cytochrome c oxidase (CO) activity, and a 5-fold increase in the concentration of hydrogen peroxide secreted into their culture medium compared with cells with no alterations in CO I mRNA processing. Our findings suggest that alterations in mt CO I processing may play a role in the neoplastic conversion of mammalian cells by ionizing radiation. | en |
| heal.journalName | Carcinogenesis | en |
| heal.journalType | peer-reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Emfietzoglou-1997-altered processing of.pdf | 304.79 kB | Adobe PDF | View/Open Request a copy |
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