Please use this identifier to cite or link to this item:
https://olympias.lib.uoi.gr/jspui/handle/123456789/23858Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Murphy, C. | en |
| dc.contributor.author | Fotsis, T. | en |
| dc.contributor.author | Pantzar, P. | en |
| dc.contributor.author | Adlercreutz, H. | en |
| dc.contributor.author | Martin, F. | en |
| dc.date.accessioned | 2015-11-24T19:36:21Z | - |
| dc.date.available | 2015-11-24T19:36:21Z | - |
| dc.identifier.issn | 0022-4731 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/23858 | - |
| dc.rights | Default Licence | - |
| dc.subject | Aged | en |
| dc.subject | Aged, 80 and over | en |
| dc.subject | Breast Neoplasms/*blood/drug therapy | en |
| dc.subject | Female | en |
| dc.subject | Gas Chromatography-Mass Spectrometry | en |
| dc.subject | Humans | en |
| dc.subject | Middle Aged | en |
| dc.subject | Quality Control | en |
| dc.subject | Tamoxifen/analogs & derivatives/*blood/therapeutic use | en |
| dc.title | Analysis of tamoxifen and its metabolites in human plasma by gas chromatography-mass spectrometry (GC-MS) using selected ion monitoring (SIM) | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/3586671 | - |
| heal.identifier.secondary | http://ac.els-cdn.com/0022473187900069/1-s2.0-0022473187900069-main.pdf?_tid=034f211dad4ba124a1a01b2f9c372af0&acdnat=1332753113_56eebce80ec94bf9c3b157d943898f0c | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.publicationDate | 1987 | - |
| heal.abstract | A method for the analysis of tamoxifen and its metabolites in plasma from tamoxifen treated breast cancer patients, by capillary GC-MS using selected ion monitoring has been developed. Metabolite extraction was carried out on a Sep-pak C18 cartridge and metabolite purification by selective ion exchange chromatographic steps. Satisfactory recovery of radioactive standards through the extraction and purification steps was obtained. The method was shown to be accurate and precise with precision coefficient of variation values ranging from 4.3-11% for tamoxifen and its metabolites. Tamoxifen, 4-hydroxytamoxifen, metabolite Y and N-desmethyltamoxifen were identified with certainty in patient plasma on the basis of GC relative retention times and mass spectral comparison with authentic standards; because of their low abundance in plasma cis-metabolite E and 3,4-dihydroxytamoxifen could only be tentatively identified but identical GC behaviour and a satisfactory comparison of the abundance of key fragment ions was achieved. The tamoxifen and metabolite concentration ranges (ng X ml-1) in the group of patients who received 40 or 80 ng tamoxifen for 14 days were tamoxifen, 307-745; N-desmethyltamoxifen, 185-491; 4-hydroxytamoxifen, 1.4-2.5; 3,4-dihydroxytamoxifen, 0.7-2.0; metabolite Y, 19.0-112; and metabolite E1, 0.9-2.0. | en |
| heal.journalName | J Steroid Biochem | en |
| heal.journalType | peer-reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Murphy-1987-Analysis of tamoxife.pdf | 956.88 kB | Adobe PDF | View/Open Request a copy |
This item is licensed under a Creative Commons License
