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https://olympias.lib.uoi.gr/jspui/handle/123456789/23453Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Tavoulari, S. | en |
| dc.contributor.author | Frillingos, S. | en |
| dc.contributor.author | Karatza, P. | en |
| dc.contributor.author | Messinis, I. E. | en |
| dc.contributor.author | Seferiadis, K. | en |
| dc.date.accessioned | 2015-11-24T19:32:47Z | - |
| dc.date.available | 2015-11-24T19:32:47Z | - |
| dc.identifier.issn | 0268-1161 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/23453 | - |
| dc.rights | Default Licence | - |
| dc.subject | Animals | en |
| dc.subject | Antibodies/pharmacology | en |
| dc.subject | Cells, Cultured | en |
| dc.subject | Female | en |
| dc.subject | Follicle Stimulating Hormone/secretion | en |
| dc.subject | Gonadal Hormones | en |
| dc.subject | Humans | en |
| dc.subject | Luteinizing Hormone/secretion | en |
| dc.subject | Male | en |
| dc.subject | Peptide Fragments/genetics/immunology/metabolism/*pharmacology | en |
| dc.subject | Pichia/metabolism | en |
| dc.subject | Pituitary Gland/cytology/secretion | en |
| dc.subject | Protein Structure, Tertiary/physiology | en |
| dc.subject | Proteins/antagonists & inhibitors/*pharmacology | en |
| dc.subject | Rats | en |
| dc.subject | Rats, Wistar | en |
| dc.subject | Recombinant Proteins/genetics/immunology/metabolism/pharmacology | en |
| dc.subject | Serum Albumin/genetics/immunology/metabolism/*pharmacology | en |
| dc.title | The recombinant subdomain IIIB of human serum albumin displays activity of gonadotrophin surge-attenuating factor | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.primary | 10.1093/humrep/deh187 | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/15016777 | - |
| heal.identifier.secondary | http://humrep.oxfordjournals.org/content/19/4/849.full.pdf | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.publicationDate | 2004 | - |
| heal.abstract | BACKGROUND: Gonadotrophin surge-attenuating factor (GnSAF) is an as yet unidentified ovarian factor that acts on the pituitary to attenuate the pre-ovulatory LH surge. In a previous study, GnSAF bioactivity was proposed to derive, at least in part, from a C-terminal domain (95peptide) of human serum albumin (HSA). METHODS AND RESULTS: We employ here the expression-secretion system of Pichia pastoris to produce and assay selected recombinant polypeptides of HSA for GnSAF activity. We show that the C-terminal 95peptide of HSA (residues 490-585; subdomain IIIB) can be expressed from P.pastoris in secreted form and supernatants from clones expressing this polypeptide reduce the GnRH-induced LH secretion of primary rat pituitary cultures by 50-82%. When expressed in the same system, HSA domain III (residues 381-585) or full-length HSA (residues 1-585) are inactive. The bioactive subdomain IIIB is also separable from either domain III or full-length HSA on Blue Sepharose chromatography. CONCLUSIONS: Taken together, the findings highlight the putative importance of HSA subdomain IIIB as a GnSAF-bioactive entity and introduce a unique experimental tool to engineer this molecule for structure-function analysis. | en |
| heal.journalName | Hum Reprod | en |
| heal.journalType | peer-reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Tavoulari-2004-The recombinant subd.pdf | 250.35 kB | Adobe PDF | View/Open Request a copy |
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