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https://olympias.lib.uoi.gr/jspui/handle/123456789/23146Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Kolettas, E. | en |
| dc.contributor.author | Muir, H. I. | en |
| dc.contributor.author | Barrett, J. C. | en |
| dc.contributor.author | Hardingham, T. E. | en |
| dc.date.accessioned | 2015-11-24T19:30:40Z | - |
| dc.date.available | 2015-11-24T19:30:40Z | - |
| dc.identifier.issn | 1462-0324 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/23146 | - |
| dc.rights | Default Licence | - |
| dc.subject | Aggrecans | en |
| dc.subject | Animals | en |
| dc.subject | Antimetabolites, Antineoplastic/pharmacology | en |
| dc.subject | Apoptosis/drug effects/immunology | en |
| dc.subject | Azacitidine/pharmacology | en |
| dc.subject | Biglycan | en |
| dc.subject | Cell Line, Transformed | en |
| dc.subject | Cell Survival/immunology | en |
| dc.subject | Chondrocytes/*cytology/*immunology | en |
| dc.subject | Collagen Type II/genetics | en |
| dc.subject | Collagen Type IX/genetics | en |
| dc.subject | Cricetinae | en |
| dc.subject | *Extracellular Matrix Proteins | en |
| dc.subject | Fetus/cytology | en |
| dc.subject | Gene Expression/drug effects/immunology | en |
| dc.subject | High Mobility Group Proteins/*genetics/immunology | en |
| dc.subject | Insulin-Like Growth Factor I/*pharmacology | en |
| dc.subject | Interleukin-1/*pharmacology | en |
| dc.subject | Lectins, C-Type | en |
| dc.subject | Mesocricetus | en |
| dc.subject | Phenotype | en |
| dc.subject | Proteins/genetics | en |
| dc.subject | Proteoglycans/genetics | en |
| dc.subject | RNA, Messenger/analysis | en |
| dc.subject | SOX9 Transcription Factor | en |
| dc.subject | Transcription Factors/*genetics/immunology | en |
| dc.title | Chondrocyte phenotype and cell survival are regulated by culture conditions and by specific cytokines through the expression of Sox-9 transcription factor | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/11600745 | - |
| heal.identifier.secondary | http://rheumatology.oxfordjournals.org/content/40/10/1146.full.pdf | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.publicationDate | 2001 | - |
| heal.abstract | OBJECTIVE: To investigate the effects of culture conditions, serum and specific cytokines such as insulin-like growth factor (IGF) 1 and interleukin (IL) 1alpha on phenotype and cell survival in cultures of Syrian hamster embryonic chondrocyte-like cells (DES4(+).2). METHODS: Proteins and RNA extracted from subconfluent and confluent early- and late-passage DES4(+).2 cells cultured in the presence or absence of serum and IL-1alpha or IGF-1 or both cytokines together were analysed for the expression of chondrocyte-specific genes and for the chondrogenic transcription factor Sox-9 by Western and Northern blotting. Apoptosis was assessed by agarose gel electrophoresis of labelled low-molecular weight DNA extracted from DES4(+).2 cells and another Syrian hamster embryonic chondrocyte-like cell line, 10W(+).1, cultured under the different conditions and treatments. RESULTS: Early passage DES4(+).2 cells expressed chondrocyte-specific molecules such as collagen types alpha1(II) and alpha1(IX), aggrecan, biglycan and link protein and collagen types alpha1(I) and alpha1(X) mRNAs, suggesting a prehypertrophic chondrocyte-like phenotype. The expression of all genes investigated was cell density- and serum-dependent and was low to undetectable in cell populations from later passages. Early-passage DES4(+).2 and 10W(+).1 cells survived when cultured at low cell density, but died by apoptosis when cultured at high cell density in the absence of serum or IGF-1. IGF-1 and IL-1alpha had opposite and antagonistic effects on the chondrocyte phenotype and survival. Whereas IL-1alpha acting alone suppressed cartilage-specific gene expression without significantly affecting cell survival, IGF-1 increased the steady-state mRNA levels and relieved the IL-1alpha-induced suppression of all the chondrocyte-specific genes investigated; it also enhanced chondrocyte survival. Suppression of the chondrocyte phenotype by the inflammatory cytokine IL-1alpha correlated with marked down-regulation of the transcription factor Sox-9, which was relieved by IGF-1. The expression of the Sox9 gene was closely correlated with the expression of the chondrocyte-specific genes under all conditions and treatments. CONCLUSIONS: The results suggest that the effects of cartilage anabolic and catabolic cytokines IGF-1 and IL-1alpha on the expression of the chondrocyte phenotype are mediated by Sox-9. As Sox-9 appears to be essential for matrix production, the potent effect of IL-1alpha in suppressing Sox-9 expression may limit the ability of cartilage to repair during inflammatory joint diseases. | en |
| heal.journalName | Rheumatology (Oxford) | en |
| heal.journalType | peer-reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Kolettas-2001-Chondrocyte phenotyp.pdf | 347.55 kB | Adobe PDF | View/Open Request a copy |
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