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https://olympias.lib.uoi.gr/jspui/handle/123456789/23039Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Tzavaras, T. | en |
| dc.contributor.author | Kalogera, C. | en |
| dc.contributor.author | Eftaxia, S. | en |
| dc.contributor.author | Saragosti, S. | en |
| dc.contributor.author | Pagoulatos, G. N. | en |
| dc.date.accessioned | 2015-11-24T19:30:12Z | - |
| dc.date.available | 2015-11-24T19:30:12Z | - |
| dc.identifier.issn | 0006-3002 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/23039 | - |
| dc.rights | Default Licence | - |
| dc.subject | 3T3 Cells | en |
| dc.subject | Animals | en |
| dc.subject | Base Sequence | en |
| dc.subject | Cell Transformation, Viral/*genetics | en |
| dc.subject | DNA Probes | en |
| dc.subject | Genes, Reporter | en |
| dc.subject | Mice | en |
| dc.subject | Plasmids | en |
| dc.subject | *Promoter Regions, Genetic | en |
| dc.subject | Recombination, Genetic | en |
| dc.subject | *Repetitive Sequences, Nucleic Acid | en |
| dc.subject | Restriction Mapping | en |
| dc.subject | *Retroelements | en |
| dc.subject | Reverse Transcriptase Polymerase Chain Reaction | en |
| dc.subject | Simian virus 40/*genetics | en |
| dc.subject | Transfection | en |
| dc.title | Clone-specific high-frequency retrotransposition of a recombinant virus containing a VL30 promoter in SV40-transformed NIH3T3 cells | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/9804952 | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.publicationDate | 1998 | - |
| heal.abstract | A recombinant virus, containing the promoter of a VL30 LTR and tagged with the neomycin gene as a selection and indicator marker, was constructed to investigate transposition events in NIH3T3 cells after SV40 transformation. This retroviral construct was transfected into psi/CRE packaging cells, and pseudovirions were used to infect NIH3T3 cells. Clones resistant to G418 bearing single-copy integrations of the recombinant virus were isolated and transformed by SV40 virus. Transpositions were detected through RFLPs with a neomycin probe and 'retrotransposition' was further confirmed by inverse PCR and DNA sequencing of transposed and parental copies. We found that: (1) retrotransposition of this recombinant virus occurred with a high frequency in a parental clone transformed with SV40 virus suggesting that the frequency of retrotransposition depended on the initial site of provirus integration; (2) the transposition frequency was independent of the transcription level of the recombinant construct; and (3) analysis of transposition-positive transformants showed that the high transposition frequency appeared to be associated with the induction of endogenous reverse transcriptases. | en |
| heal.journalName | Biochim Biophys Acta | en |
| heal.journalType | peer-reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Pagoulatos-1998-clone specific high frequency.pdf | 263.59 kB | Adobe PDF | View/Open Request a copy |
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