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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/22755
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dc.contributor.authorPliaka, V.en
dc.contributor.authorKyriakopoulou, Z.en
dc.contributor.authorTsakogiannis, D.en
dc.contributor.authorRuether, I. G.en
dc.contributor.authorGartzonika, C.en
dc.contributor.authorLevidiotou-Stefanou, S.en
dc.contributor.authorKrikelis, A.en
dc.contributor.authorMarkoulatos, P.en
dc.date.accessioned2015-11-24T19:26:45Z-
dc.date.available2015-11-24T19:26:45Z-
dc.identifier.issn1435-4373-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/22755-
dc.rightsDefault Licence-
dc.subjectCell Lineen
dc.subjectGenome, Viralen
dc.subjectHumansen
dc.subjectKineticsen
dc.subject*Mutationen
dc.subjectPoliovirus/genetics/*growth & development/isolation & purification/*pathogenicityen
dc.subject*Poliovirus Vaccine, Oralen
dc.subjectRNA, Viral/geneticsen
dc.subject*Recombination, Geneticen
dc.subjectSequence Analysis, DNAen
dc.subjectTemperatureen
dc.subjectVaccines, Attenuateden
dc.titleCorrelation of mutations and recombination with growth kinetics of poliovirus vaccine strainsen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.primary10.1007/s10096-010-1033-9-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/20820837-
heal.identifier.secondaryhttp://www.springerlink.com/content/kr76255vh76257h0/fulltext.pdf-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate2010-
heal.abstractAttenuated strains of Sabin poliovirus vaccine replicate in the human gut and, in rare cases, may cause vaccine-associated paralytic poliomyelitis (VAPP). The genetic instability of Sabin strains constitutes one of the main causes of VAPP, a disease that is most frequently associated with type 3 and type 2 Sabin strains, and more rarely with type 1 Sabin strains. In the present study, the growth phenotype of eight oral poliovirus vaccine (OPV) isolates (two non-recombinants and six recombinants), as well as of Sabin vaccine strains, was evaluated using two different assays, the reproductive capacity at different temperatures (Rct) test and the one-step growth curve test in Hep-2 cells at two different temperatures (37 degrees C and 40 degrees C). The growth phenotype of isolates was correlated with genomic modifications in order to identify the determinants and mechanisms of reversion towards neurovirulence. All of the recombinant OPV isolates showed a thermoresistant phenotype in the Rct test. Moreover, both recombinant Sabin-3 isolates showed significantly higher viral yield than Sabin 3 vaccine strain at 37 degrees C and 40 degrees C in the one-step growth curve test. All of the OPV isolates displayed mutations at specific sites of the viral genome, which are associated with the attenuated and temperature-sensitive phenotype of Sabin strains. The results showed that both mutations and recombination events could affect the phenotype traits of Sabin derivatives and may lead to the reversion of vaccinal strains to neurovirulent ones. The use of phenotypic markers along with the genomic analysis may shed additional light on the molecular determinants of the reversed neurovirulent phenotype of Sabin derivatives.en
heal.journalNameEur J Clin Microbiol Infect Disen
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
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