1. Repository of OAI
  2. ΑΠΟΘΕΤΗΡΙΟ "ΟΛΥΜΠΙΑΣ"
  3. Σχολή Επιστημών Υγείας
  4. Τμήμα Ιατρικής
  5. Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ
Ελληνικά   English  
Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/22341
Full metadata record
DC FieldValueLanguage
dc.contributor.authorMohanam, S.en
dc.contributor.authorJasti, S. L.en
dc.contributor.authorKondraganti, S. R.en
dc.contributor.authorChandrasekar, N.en
dc.contributor.authorLakka, S. S.en
dc.contributor.authorKin, Y.en
dc.contributor.authorFuller, G. N.en
dc.contributor.authorYung, A. W.en
dc.contributor.authorKyritsis, A. P.en
dc.contributor.authorDinh, D. H.en
dc.contributor.authorOlivero, W. C.en
dc.contributor.authorGujrati, M.en
dc.contributor.authorAli-Osman, F.en
dc.contributor.authorRao, J. S.en
dc.date.accessioned2015-11-24T19:23:36Z-
dc.date.available2015-11-24T19:23:36Z-
dc.identifier.issn0950-9232-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/22341-
dc.rightsDefault Licence-
dc.subjectAnimalsen
dc.subjectBlotting, Northern/methodsen
dc.subjectBlotting, Western/methodsen
dc.subjectBrain/pathologyen
dc.subjectBrain Neoplasms/*pathologyen
dc.subjectCathepsin B/genetics/metabolism/*physiologyen
dc.subject*Down-Regulationen
dc.subjectGene Expressionen
dc.subjectGlioblastoma/*pathologyen
dc.subjectHumansen
dc.subjectInjectionsen
dc.subjectMiceen
dc.subjectMice, Nudeen
dc.subject*Neoplasm Invasivenessen
dc.subjectNeoplasm Transplantationen
dc.subjectRNA, Messengeren
dc.subjectRatsen
dc.subjectRats, Sprague-Dawleyen
dc.subjectSpheroids, Cellular/pathologyen
dc.subjectTransfectionen
dc.subjectTumor Cells, Cultureden
dc.titleDown-regulation of cathepsin B expression impairs the invasive and tumorigenic potential of human glioblastoma cellsen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.primary10.1038/sj.onc.1204480-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/11439329-
heal.identifier.secondaryhttp://www.nature.com/onc/journal/v20/n28/pdf/1204480a.pdf-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate2001-
heal.abstractIncreases in abundance of cathepsin B transcript and protein correlate with increases in tumor grade and alterations in subcellular localization and activity of cathepsin B. The enzyme is able to degrade the components of the extracellular matrix (ECM) and activate other proteases capable of degrading ECM. To investigate the role played by this protease in the invasion of brain tumor cells, we transfected SNB19 human glioblastoma cells with a plasmid containing cathepsin B cDNA in antisense orientation. Control cells were transfected with vector alone. Clones expressing antisense cathepsin B cDNA exhibited significant reductions in cathepsin B mRNA, enzyme activity and protein compared to controls. Matrigel Invasion assay showed that the antisense-transfected cells had a markedly diminished invasiveness compared with controls. When tumor spheroids containing antisense transfected SNB19 cells expressing reduced cathepsin B were co-cultured with fetal rat brain aggregates, invasion of fetal rat brain aggregates was significantly reduced. Green Fluorescent Protein (GFP) expressing parental cells and antisense transfectants were generated for detection in mouse brain tissue without any post-chemical treatment. Intracerebral injection of SNB19 stable antisense transfectants resulted in reduced tumor formation in nude mice. These results strongly support a role for cathepsin B in the invasiveness of human glioblastoma cells and suggest cathepsin B antisense may prove useful in cancer therapy.en
heal.journalNameOncogeneen
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ

Show simple item record
Files in This Item:
File Description SizeFormat 
Mohanam-2001-Down-regulation of c.pdf403.15 kBAdobe PDFView/Open    Request a copy
Show simple item record  


This item is licensed under a Creative Commons License Creative Commons