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https://olympias.lib.uoi.gr/jspui/handle/123456789/22339Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Mohan, P. M. | en |
| dc.contributor.author | Lakka, S. S. | en |
| dc.contributor.author | Mohanam, S. | en |
| dc.contributor.author | Kin, Y. | en |
| dc.contributor.author | Sawaya, R. | en |
| dc.contributor.author | Kyritsis, A. P. | en |
| dc.contributor.author | Nicolson, G. L. | en |
| dc.contributor.author | Rao, J. S. | en |
| dc.date.accessioned | 2015-11-24T19:23:36Z | - |
| dc.date.available | 2015-11-24T19:23:36Z | - |
| dc.identifier.issn | 0262-0898 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/22339 | - |
| dc.rights | Default Licence | - |
| dc.subject | Collagen | en |
| dc.subject | DNA, Antisense/*pharmacology | en |
| dc.subject | Down-Regulation/*drug effects | en |
| dc.subject | Drug Combinations | en |
| dc.subject | Glioblastoma/metabolism/*pathology | en |
| dc.subject | Humans | en |
| dc.subject | Laminin | en |
| dc.subject | Neoplasm Invasiveness/physiopathology/*prevention & control | en |
| dc.subject | Neoplasm Proteins/*biosynthesis/genetics/physiology | en |
| dc.subject | Protein Biosynthesis/*drug effects | en |
| dc.subject | Proteoglycans | en |
| dc.subject | RNA, Antisense/biosynthesis/genetics | en |
| dc.subject | RNA, Messenger/metabolism | en |
| dc.subject | RNA, Neoplasm/metabolism | en |
| dc.subject | Receptors, Cell Surface/*biosynthesis/genetics/physiology | en |
| dc.subject | Receptors, Urokinase Plasminogen Activator | en |
| dc.subject | Transfection | en |
| dc.subject | Tumor Cells, Cultured | en |
| dc.title | Downregulation of the urokinase-type plasminogen activator receptor through inhibition of translation by antisense oligonucleotide suppresses invasion of human glioblastoma cells | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/10845561 | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.publicationDate | 1999 | - |
| heal.abstract | We previously showed that downregulation of the urokinase-type plasminogen activator receptor (uPAR) in the SNB19 human glioblastoma cell line by the stable transfection of a plasmid expressing a 300 bp antisense sequence to the 5' end of the uPAR gene produced a decrease in the amount of target mRNA. In a more recent study, we found that adenovirus-mediated transduction (Ad-uPAR) of the same uPAR antisense gene construct in SNB19 cells also downregulated uPAR protein levels. We report here that Ad-uPAR-transfected SNB19 cells produced the same amounts of target uPAR mRNA but significantly less protein by in vitro translation and by in situ [35S] labeling compared to Ad-CMV vector-transfected and mock-transfected cells. This antisense construct also inhibited glioblastoma cell invasion confirming previous results. We conclude that downregulation of uPAR by this antisense gene construct results from inhibition of protein translation. | en |
| heal.journalName | Clin Exp Metastasis | en |
| heal.journalType | peer-reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ | |
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