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  4. Τμήμα Ιατρικής
  5. Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ
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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/22319
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dc.contributor.authorKourmouli, N.en
dc.contributor.authorTheodoropoulos, P. A.en
dc.contributor.authorDialynas, G.en
dc.contributor.authorBakou, A.en
dc.contributor.authorPolitou, A. S.en
dc.contributor.authorCowell, I. G.en
dc.contributor.authorSingh, P. B.en
dc.contributor.authorGeorgatos, S. D.en
dc.date.accessioned2015-11-24T19:23:30Z-
dc.date.available2015-11-24T19:23:30Z-
dc.identifier.issn0261-4189-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/22319-
dc.rightsDefault Licence-
dc.subjectAcetylationen
dc.subjectAnimalsen
dc.subjectBinding Sitesen
dc.subjectBinding, Competitiveen
dc.subjectCHO Cellsen
dc.subjectCell Lineen
dc.subjectCell Nucleus/metabolismen
dc.subjectChromatin/metabolismen
dc.subjectChromosomal Proteins, Non-Histone/chemistry/genetics/*metabolismen
dc.subjectChromosomes/metabolismen
dc.subjectCricetinaeen
dc.subjectCytoplasm/metabolismen
dc.subject*DNA-Binding Proteinsen
dc.subjectDetergents/pharmacologyen
dc.subjectDose-Response Relationship, Drugen
dc.subjectElectrophoresis, Polyacrylamide Gelen
dc.subjectFluorescent Antibody Technique, Indirecten
dc.subjectGlutathione Transferase/metabolismen
dc.subjectHeLa Cellsen
dc.subjectHumansen
dc.subjectImmunoblottingen
dc.subjectKineticsen
dc.subjectLaminsen
dc.subjectMembrane Proteins/metabolismen
dc.subjectMiceen
dc.subjectMicroinjectionsen
dc.subjectMitosisen
dc.subjectMutationen
dc.subjectNuclear Envelope/*metabolismen
dc.subjectNuclear Proteins/metabolismen
dc.subjectOctoxynol/pharmacologyen
dc.subjectProtein Bindingen
dc.subjectProtein Transporten
dc.subjectRecombinant Fusion Proteins/physiologyen
dc.titleDynamic associations of heterochromatin protein 1 with the nuclear envelopeen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.primary10.1093/emboj/19.23.6558-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/11101528-
heal.identifier.secondaryhttp://www.nature.com/emboj/journal/v19/n23/pdf/7593468a.pdf-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate2000-
heal.abstractTo study the dynamics of mammalian HP1 proteins we have microinjected recombinant forms of mHP1alpha, M31 and M32 into the cytoplasm of living cells. As could be expected from previous studies, the three fusion proteins were efficiently transported into the nucleus and targeted specific chromatin areas. However, before incorporation into these areas the exogenous proteins accumulated in a peripheral zone and associated closely with the nuclear envelope. This transient association did not occur when the cells were treated with deacetylase inhibitors, indicating an acetylation-inhibited interaction. In line with these observations, recombinant HP1 proteins exhibited saturable binding to purified nuclear envelopes and stained the nuclei of detergent-permeabilized cells in a rim-like fashion. Competition experiments with various M31 mutants allowed mapping of the nuclear envelope-binding site within an N-terminal region that includes the chromodomain. A His(6)-tagged peptide representing this region inhibited recruitment of LAP2beta and B-type lamins around the surfaces of condensed chromosomes, suggesting involvement of HP1 proteins in nuclear envelope reassembly.en
heal.journalNameEMBO Jen
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
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