Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/22181
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dc.contributor.authorGeorgiou, I.en
dc.contributor.authorSyrrou, M.en
dc.contributor.authorStefanidis, K.en
dc.contributor.authorKonstantelli, M.en
dc.contributor.authorLolis, D.en
dc.date.accessioned2015-11-24T19:22:40Z-
dc.date.available2015-11-24T19:22:40Z-
dc.identifier.issn0303-4569-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/22181-
dc.rightsDefault Licence-
dc.subjectCell Separation/*methodsen
dc.subject*Centrifugation, Density Gradienten
dc.subject*Chromomycin A3en
dc.subjectDNA/analysisen
dc.subjectFertilization in Vitroen
dc.subject*Fluorescent Dyesen
dc.subjectHumansen
dc.subject*Infertility, Maleen
dc.subjectMaleen
dc.subjectPovidoneen
dc.subjectSilicon Dioxideen
dc.subjectSperm Motilityen
dc.subjectSpermatozoa/*chemistryen
dc.subjectStaining and Labelingen
dc.titleEffect of Percoll gradient and swim-up preparation on the chromomycin A3 staining of normal and abnormal semen samplesen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/9629431-
heal.identifier.secondaryhttp://onlinelibrary.wiley.com/store/10.1111/j.1439-0272.1998.tb01154.x/asset/j.1439-0272.1998.tb01154.x.pdf?v=1&t=h0dir0wa&s=c50f31c1622f799b7565229594fb3e653017f0ce-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate1998-
heal.abstractThe protamination of spermatozoa recovered by Percoll gradient and swim-up was investigated by means of chromomycin A3 (CMA3) staining. A total of 34 semen samples from patients undergoing IVF treatment were divided in two groups: normal (A) and oligoasthenozoospermic (B). Samples were divided in fractions, subjected to both techniques of preparation, and stained by CMA3. The percentage of CMA3 positive spermatozoa recovered by Percoll was comparable to swim-up in the normal group. In the abnormal group Percoll resulted in significantly lower CMA3 percentage. It is concluded that the degree of protamination in recovered spermatozoa is influenced by the technique of preparation.en
heal.journalNameAndrologiaen
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ

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