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  5. Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ
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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/21693
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dc.contributor.authorSimitsopoulou, M.en
dc.contributor.authorRoilides, E.en
dc.contributor.authorLikartsis, C.en
dc.contributor.authorIoannidis, J.en
dc.contributor.authorOrfanou, A.en
dc.contributor.authorPaliogianni, F.en
dc.contributor.authorWalsh, T. J.en
dc.date.accessioned2015-11-24T19:16:39Z-
dc.date.available2015-11-24T19:16:39Z-
dc.identifier.issn0066-4804-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/21693-
dc.rightsDefault Licence-
dc.subjectAntifungal Agents/*pharmacologyen
dc.subjectAspergillus fumigatus/*immunologyen
dc.subjectCells, Cultureden
dc.subjectChemokine CCL2/biosynthesisen
dc.subjectChemokine CCL3en
dc.subjectChemokine CCL4en
dc.subjectDNA, Complementary/biosynthesis/geneticsen
dc.subjectGene Expression Regulation/*drug effectsen
dc.subjectHumansen
dc.subjectHyphae/immunologyen
dc.subjectImmunity, Innate/*drug effects/*geneticsen
dc.subjectInterleukin-10/biosynthesisen
dc.subjectInterleukin-12/biosynthesisen
dc.subjectInterleukin-1beta/biosynthesisen
dc.subjectMacrophage Inflammatory Proteins/biosynthesisen
dc.subjectMonocytes/drug effects/*immunologyen
dc.subjectOligonucleotide Array Sequence Analysisen
dc.subjectPyrimidines/*pharmacologyen
dc.subjectRNA/biosynthesis/geneticsen
dc.subjectReverse Transcriptase Polymerase Chain Reactionen
dc.subjectTriazoles/*pharmacologyen
dc.subjectTumor Necrosis Factor-alpha/biosynthesisen
dc.titleExpression of immunomodulatory genes in human monocytes induced by voriconazole in the presence of Aspergillus fumigatusen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.primary10.1128/AAC.01095-06-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/17178797-
heal.identifier.secondaryhttp://aac.asm.org/content/51/3/1048.full.pdf-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate2007-
heal.abstractWe assessed the effect of voriconazole (VRC) on the expression and release of selected cytokines and chemokines in the THP-1 human monocytic cell line in response to Aspergillus fumigatus hyphal fragments (HF) by cDNA microarray analysis, reverse transcriptase (RT) PCR, and enzyme-linked immunosorbent assay. Stimulation of THP-1 cells by HF alone caused a significant up-regulation of CCL4 (MIP1B) and CCL16, while CCL2 (MCP1) was down-regulated. By comparison, in the presence of VRC, a large number of genes such as CCL3 (MIP1A), CCL4 (MIP1B), CCL5 (RANTES), CCL7 (MCP3), CCL11 (EOTAXIN), CCL15 (MIP1Delta), CXCL6, and CXCL13 were strongly up-regulated in THP-1 cells challenged by HF, whereas CCL20 (MIP3A) and CCL21 (MIP2) were down-regulated. Among five genes differentially expressed in THP-1 cells, IL12A, IL12B, and IL-16 were down-regulated whereas IL-11 and TGFB1 were significantly up-regulated in the presence of VRC. The inflammation-related genes IFNgamma, IL1R1, and TNFA were also up-regulated in THP-1 cells exposed to HF only in the presence of VRC. RT-PCR of four selected genes validated the results of microarrays. The release of interleukin 1beta (IL-1beta) and IL-12 was significantly increased from monocytes stimulated either by HF alone (P < 0.05) or in the presence of VRC (P < 0.01 and P < 0.05, respectively). In contrast, tumor necrosis factor alpha release from monocytes was enhanced only in the presence of VRC (P < 0.01). The chemokines monocyte chemoattractant protein 1 and macrophage inflammatory protein 1beta were decreased under both conditions (P < 0.01). These results demonstrate that in the presence of VRC, HF induces a more pronounced profile of gene expression in THP-1 cells than HF alone, potentially leading to more-efficient host resistance to A. fumigatus.en
heal.journalNameAntimicrob Agents Chemotheren
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
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