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DC Field | Value | Language |
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dc.contributor.author | Dova, L. | en |
dc.contributor.author | Pentheroudakis, G. | en |
dc.contributor.author | Georgiou, I. | en |
dc.contributor.author | Malamou-Mitsi, V. | en |
dc.contributor.author | Vartholomatos, G. | en |
dc.contributor.author | Fountzilas, G. | en |
dc.contributor.author | Kolaitis, N. | en |
dc.contributor.author | Kitsiou, E. | en |
dc.contributor.author | Pavlidis, N. | en |
dc.date.accessioned | 2015-11-24T19:14:41Z | - |
dc.date.available | 2015-11-24T19:14:41Z | - |
dc.identifier.issn | 0262-0898 | - |
dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/21370 | - |
dc.rights | Default Licence | - |
dc.subject | Aged | en |
dc.subject | Aged, 80 and over | en |
dc.subject | Base Sequence | en |
dc.subject | DNA Primers | en |
dc.subject | DNA, Neoplasm/genetics | en |
dc.subject | Female | en |
dc.subject | *Gene Expression Profiling | en |
dc.subject | *Genes, erbB-1 | en |
dc.subject | Humans | en |
dc.subject | Immunohistochemistry | en |
dc.subject | Introns | en |
dc.subject | Male | en |
dc.subject | Middle Aged | en |
dc.subject | Molecular Sequence Data | en |
dc.subject | *Mutation | en |
dc.subject | Neoplasm Proteins/*genetics | en |
dc.subject | Neoplasms, Unknown Primary/*genetics | en |
dc.subject | Polymerase Chain Reaction | en |
dc.title | Global profiling of EGFR gene mutation, amplification, regulation and tissue protein expression in unknown primary carcinomas: to target or not to target? | en |
heal.type | journalArticle | - |
heal.type.en | Journal article | en |
heal.type.el | Άρθρο Περιοδικού | el |
heal.identifier.primary | 10.1007/s10585-007-9055-0 | - |
heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/17390112 | - |
heal.identifier.secondary | http://www.springerlink.com/content/u7872w40578304q4/fulltext.pdf | - |
heal.language | en | - |
heal.access | campus | - |
heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
heal.publicationDate | 2007 | - |
heal.abstract | INTRODUCTION: Epidermal growth factor receptor (EGFR) signalling contributes to malignant transformation and survival. We studied molecular predictors of benefit from EGFR-modulating therapies in patients with cancer of unknown primary (CUP). MATERIALS AND METHODS: Tumours from paraffin-embedded biopsies of 50 patients with CUP were stained for EGFR protein by immunohistochemistry. Polymerase chain reaction amplification, single-strand conformational polymorphism and direct sequencing were used to study EGFR intron 1 cytosine-adenosine (CA) repeat length as well as exon 18, 19, 21 activating mutations and amplification. RESULTS: Thirty-seven tumours (74%) expressed EGFR protein but only six (12%) strongly. Regarding intron 1 CA repeat length, we detected five alleles with CA repeat numbers 16-20, allele 16 being the most common (39%). All samples were heterozygous, the commonest genotype consisting of 16/18 dinucleotides (78%). Five samples had three intron 1 alleles and were associated with EGFR overexpression in 40% of cases. There was no evidence of EGFR exon 18, 19, 21 amplification. Two mutations were detected: Exon 21 2508 C > T, a silent nucleotide polymorphism (R836R) and a G > A substitution in sequences flanking exon 19 (IVS19 + 24G > A) resulting in aberrant mRNA splicing. Neither EGFR protein expression nor CA repeat length were prognostic factors for survival. CONCLUSIONS: Our data depict absence of molecular predictors of benefit from EGFR modulation in patients with CUP. Study of its molecular pathophysiology and targeting other molecular pathways may be warranted instead. | en |
heal.journalName | Clin Exp Metastasis | en |
heal.journalType | peer-reviewed | - |
heal.fullTextAvailability | TRUE | - |
Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ |
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Dova-2007-Global profiling of.pdf | 276.42 kB | Adobe PDF | View/Open Request a copy |
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