Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/21370
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dc.contributor.authorDova, L.en
dc.contributor.authorPentheroudakis, G.en
dc.contributor.authorGeorgiou, I.en
dc.contributor.authorMalamou-Mitsi, V.en
dc.contributor.authorVartholomatos, G.en
dc.contributor.authorFountzilas, G.en
dc.contributor.authorKolaitis, N.en
dc.contributor.authorKitsiou, E.en
dc.contributor.authorPavlidis, N.en
dc.date.accessioned2015-11-24T19:14:41Z-
dc.date.available2015-11-24T19:14:41Z-
dc.identifier.issn0262-0898-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/21370-
dc.rightsDefault Licence-
dc.subjectAgeden
dc.subjectAged, 80 and overen
dc.subjectBase Sequenceen
dc.subjectDNA Primersen
dc.subjectDNA, Neoplasm/geneticsen
dc.subjectFemaleen
dc.subject*Gene Expression Profilingen
dc.subject*Genes, erbB-1en
dc.subjectHumansen
dc.subjectImmunohistochemistryen
dc.subjectIntronsen
dc.subjectMaleen
dc.subjectMiddle Ageden
dc.subjectMolecular Sequence Dataen
dc.subject*Mutationen
dc.subjectNeoplasm Proteins/*geneticsen
dc.subjectNeoplasms, Unknown Primary/*geneticsen
dc.subjectPolymerase Chain Reactionen
dc.titleGlobal profiling of EGFR gene mutation, amplification, regulation and tissue protein expression in unknown primary carcinomas: to target or not to target?en
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.primary10.1007/s10585-007-9055-0-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/17390112-
heal.identifier.secondaryhttp://www.springerlink.com/content/u7872w40578304q4/fulltext.pdf-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate2007-
heal.abstractINTRODUCTION: Epidermal growth factor receptor (EGFR) signalling contributes to malignant transformation and survival. We studied molecular predictors of benefit from EGFR-modulating therapies in patients with cancer of unknown primary (CUP). MATERIALS AND METHODS: Tumours from paraffin-embedded biopsies of 50 patients with CUP were stained for EGFR protein by immunohistochemistry. Polymerase chain reaction amplification, single-strand conformational polymorphism and direct sequencing were used to study EGFR intron 1 cytosine-adenosine (CA) repeat length as well as exon 18, 19, 21 activating mutations and amplification. RESULTS: Thirty-seven tumours (74%) expressed EGFR protein but only six (12%) strongly. Regarding intron 1 CA repeat length, we detected five alleles with CA repeat numbers 16-20, allele 16 being the most common (39%). All samples were heterozygous, the commonest genotype consisting of 16/18 dinucleotides (78%). Five samples had three intron 1 alleles and were associated with EGFR overexpression in 40% of cases. There was no evidence of EGFR exon 18, 19, 21 amplification. Two mutations were detected: Exon 21 2508 C > T, a silent nucleotide polymorphism (R836R) and a G > A substitution in sequences flanking exon 19 (IVS19 + 24G > A) resulting in aberrant mRNA splicing. Neither EGFR protein expression nor CA repeat length were prognostic factors for survival. CONCLUSIONS: Our data depict absence of molecular predictors of benefit from EGFR modulation in patients with CUP. Study of its molecular pathophysiology and targeting other molecular pathways may be warranted instead.en
heal.journalNameClin Exp Metastasisen
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
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