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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/21366
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dc.contributor.authorPanayiotidis, M.en
dc.contributor.authorTsolas, O.en
dc.contributor.authorGalaris, D.en
dc.date.accessioned2015-11-24T19:14:40Z-
dc.date.available2015-11-24T19:14:40Z-
dc.identifier.issn0891-5849-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/21366-
dc.rightsDefault Licence-
dc.subjectAntioxidants/*pharmacologyen
dc.subjectAscorbic Acid/pharmacologyen
dc.subjectCalcium/*blooden
dc.subjectCatalase/pharmacologyen
dc.subjectCells, Cultureden
dc.subjectCycloheximide/pharmacologyen
dc.subjectCytochalasin B/pharmacologyen
dc.subjectDNA/drug effectsen
dc.subject*DNA Damageen
dc.subjectDehydroascorbic Acid/pharmacologyen
dc.subjectEgtazic Acid/analogs & derivatives/pharmacologyen
dc.subjectElectrophoresisen
dc.subjectGlucose Oxidase/*metabolismen
dc.subjectHumansen
dc.subjectHydrogen Peroxide/*pharmacologyen
dc.subjectKineticsen
dc.subjectLymphocytes/drug effects/*physiologyen
dc.subjectOxidative Stressen
dc.subjectVitamin E/pharmacologyen
dc.titleGlucose oxidase-produced H2O2 induces Ca2+-dependent DNA damage in human peripheral blood lymphocytesen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/10218643-
heal.identifier.secondaryhttp://ac.els-cdn.com/S0891584998002494/1-s2.0-S0891584998002494-main.pdf?_tid=c1af29f23e884486e5425c317a7da8fe&acdnat=1337848943_7de919b7451365da7e744d6597722b3a-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate1999-
heal.abstractDNA of lymphocytes from human peripheral blood was analyzed by using the single cell gel electrophoresis technique (comet assay). The cells were used either as received from the donors or after treatment with various concentrations of the H2O2-generating enzyme glucose oxidase, in order to achieve a continuous flow of H2O2. The formation of single strand breaks (SSB) was dose-related but the time course of the induction of SSB by relatively low concentrations of glucose oxidase was of a biphasic mode with a fast increase 2 to 5 min after the addition of glucose oxidase followed by a gradual decrease toward the original base level during the next 35 to 60 min. This response of the cells appears to be based on the activation of already existing defense system(s) because it was shown that H2O2 is continuously released during the reaction time and the inhibition of protein synthesis does not affect the observed pattern. Supplementation of the growth medium with various antioxidants resulted in substantial protection only when the agents were taken up by the cells. The presence of the intracellular calcium chelator BAPTA protected the cells from H2O2-induced DNA damage in a dose-dependent manner. Only at the higher rate of H2O2-generation considerable DNA damage was observed in the presence of BAPTA. These results suggest that H2O2, at low concentrations induces DNA damage through intracellular Ca2+ -mediated processes, which lead to DNA strand breaks possibly by endonuclease activation.en
heal.journalNameFree Radic Biol Meden
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
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