Please use this identifier to cite or link to this item:
https://olympias.lib.uoi.gr/jspui/handle/123456789/20952Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Tigas, S. K. | en |
| dc.contributor.author | Sunehag, A. L. | en |
| dc.contributor.author | Haymond, M. W. | en |
| dc.date.accessioned | 2015-11-24T19:11:43Z | - |
| dc.date.available | 2015-11-24T19:11:43Z | - |
| dc.identifier.issn | 0012-1797 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/20952 | - |
| dc.rights | Default Licence | - |
| dc.subject | Adult | en |
| dc.subject | Blood Glucose/*metabolism | en |
| dc.subject | Carbon Isotopes | en |
| dc.subject | Deuterium | en |
| dc.subject | Gluconeogenesis/*physiology | en |
| dc.subject | Glucose/administration & dosage/pharmacology | en |
| dc.subject | Glucose Clamp Technique | en |
| dc.subject | Glycogen/*physiology | en |
| dc.subject | Homeostasis | en |
| dc.subject | Humans | en |
| dc.subject | Isotope Labeling | en |
| dc.subject | Kinetics | en |
| dc.subject | Male | en |
| dc.subject | Reference Values | en |
| dc.subject | Time Factors | en |
| dc.title | Impact of duration of infusion and choice of isotope label on isotope recycling in glucose homeostasis | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/12401707 | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.publicationDate | 2002 | - |
| heal.abstract | The purposes of this study were to quantify the impact of the duration of infusion and choice of stable isotope of glucose on measures of glucose rate of appearance (glucose R(a)) and to determine whether the differences observed were due to tracer recycling via the glycogen pool (direct pathway) or gluconeogenesis (indirect pathway). Six healthy adult volunteers were studied on four occasions in the postabsorptive state during infusions of [1-(13)C]glucose and [6,6-(2)H(2)]glucose: 2.5-h infusion of both (A), and 2.5-h infusion of one (B) and 14.5-h infusion of the other isotope (C), and 5-h infusion of [6,6-(2)H(2)]glucose and 2.5-h infusion of [1-(13)C]glucose (D). Infusion of both isotopes for 2.5 h resulted in similar glucose R(a) values. When compared with a 14.5-h infusion, the 2.5-h glucose tracer infusion overestimated glucose R(a) by 26-35%. Glucose (13)C recycled via the Cori cycle, resulting in slower decay from the plasma pool and longer half-life of [1-(13)C]glucose compared with [6,6-(2)H(2)]glucose. There was no detectable release of [(13)C]glucose or [(2)H(2)]glucose tracer into the plasma pool after administration of glucagon. These data demonstrate that glucose R(a) varies not as a result of isotope cycling but as a result of differences in duration of isotope infusion regardless of the isotope used. This is most likely due to incomplete isotope and substrate equilibration with the 2.5-h infusion. The potential error was reduced by nearly 80% using a 5-h infusion of [6,6-(2)H(2)]glucose. These studies demonstrate that the duration of isotope infusion has significantly greater impact on quantitation of glucose R(a) than does the selection of isotope. | en |
| heal.journalName | Diabetes | en |
| heal.journalType | peer-reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ | |
Files in This Item:
There are no files associated with this item.
This item is licensed under a Creative Commons License
