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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/20944
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dc.contributor.authorHatzidaki, E.en
dc.contributor.authorNakos, G.en
dc.contributor.authorGaliatsou, E.en
dc.contributor.authorLekka, M. E.en
dc.date.accessioned2015-11-24T19:11:36Z-
dc.date.available2015-11-24T19:11:36Z-
dc.identifier.issn0006-3002-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/20944-
dc.rightsDefault Licence-
dc.subjectAdulten
dc.subjectAgeden
dc.subjectBiological Markers/metabolismen
dc.subjectBlotting, Westernen
dc.subjectCells, Cultureden
dc.subjectFemaleen
dc.subjectFlow Cytometryen
dc.subjectHumansen
dc.subjectInterferon-gamma/pharmacologyen
dc.subjectLipopolysaccharides/pharmacologyen
dc.subjectMacrophages, Alveolar/drug effects/*enzymologyen
dc.subjectMaleen
dc.subjectMiddle Ageden
dc.subjectMonocytes/drug effects/*enzymologyen
dc.subjectPhospholipases A2/*metabolismen
dc.subjectRespiratory Distress Syndrome, Adult/*enzymology/immunologyen
dc.subjectToll-Like Receptor 4/metabolismen
dc.subjectYoung Adulten
dc.titleImpaired phospholipases Aproduction by stimulated macrophages from patients with acute respiratory distress syndromeen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.primary10.1016/j.bbadis.2010.06.008-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/20600872-
heal.identifier.secondaryhttp://ac.els-cdn.com/S0925443910001183/1-s2.0-S0925443910001183-main.pdf?_tid=70b74ff24d9364656586019b61d87e85&acdnat=1333608627_a392c3698aecbd436539dbef4646de65-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate2010-
heal.abstractThe aim of this study was to investigate whether early phase of acute respiratory distress syndrome (ARDS) is associated with changes in immune response, either systemic or localized to the lung. ARDS and control mechanically ventilated patients, as well as healthy volunteers were studied. Alveolar macrophages (AMPhi) and blood monocytes (BM) were treated ex vivo with lipopolysaccharide (LPS), interferon-gamma (IFNgamma), and surfactant. Phospholipase A (PLA) activity and TLR4 expression were evaluated as markers of cell response. AMPhi from ARDS patients did not respond upon treatment with either LPS or IFN-gamma by inducing PLA production. On the contrary, upon stimulation, in control patients the intracellular PLA, (mainly cPLA) levels were increased, but secretion of PLA (mainly sPLA-IIA) was observed only after treatment with LPS. Surfactant suppressed PLA production in cells from both groups of patients. Increased relative changes of total PLA activity and an upregulation of TLR4 expression upon stimulation was observed in BM from primary ARDS, control patients and healthy volunteers. In BM from secondary ARDS patients, however, no PLA induction was observed, with a concomitant down-regulation of TLR4 expression. Cytosolic PLA, its activated form, p-cPLA, and sPLA-IIA were the predominant PLA types within the cells, while extracellularly only sPLA-IIA was identified. These results support the concept of down-regulated innate immunity in early ARDS that is compartmentalized in primary and systemic in secondary ARDS. PLA isoforms could serve as markers of the immunity status in ARDS. Finally, our data highlight the role of surfactant in controlling inflammation.en
heal.journalNameBiochim Biophys Actaen
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
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