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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/20369
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dc.contributor.authorCamus-Randon, A. M.en
dc.contributor.authorRaffalli, F.en
dc.contributor.authorBereziat, J. C.en
dc.contributor.authorMcGregor, D.en
dc.contributor.authorKonstandi, M.en
dc.contributor.authorLang, M. A.en
dc.date.accessioned2015-11-24T19:06:43Z-
dc.date.available2015-11-24T19:06:43Z-
dc.identifier.issn0041-008X-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/20369-
dc.rightsDefault Licence-
dc.subjectAnimalsen
dc.subject*Aryl Hydrocarbon Hydroxylasesen
dc.subjectBlotting, Northernen
dc.subjectBlotting, Westernen
dc.subjectCarcinogens/toxicityen
dc.subjectChloroform/*toxicityen
dc.subjectCytochrome P-450 Enzyme System/*biosynthesis/metabolismen
dc.subjectLiver/drug effects/*enzymology/pathologyen
dc.subjectMaleen
dc.subjectMiceen
dc.subjectMice, Inbred DBAen
dc.subjectMicrosomes, Liver/drug effects/enzymologyen
dc.subjectMixed Function Oxygenases/*biosynthesis/metabolismen
dc.subjectPyrazoles/toxicityen
dc.subjectThioacetamide/*toxicityen
dc.subjectXenobiotics/*metabolismen
dc.titleLiver injury and expression of cytochromes P450: evidence that regulation of CYP2A5 is different from that of other major xenobiotic metabolizing CYP enzymesen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.primary10.1006/taap.1996.0107-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/8658503-
heal.identifier.secondaryhttp://ac.els-cdn.com/S0041008X96901076/1-s2.0-S0041008X96901076-main.pdf?_tid=9cfaff052189b8f26c4a8485756bed08&acdnat=1332759322_e586b481b7b74d89bb36d86366526db0-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate1996-
heal.abstractThe purpose of this study was to find out how liver injury caused by two well-known hepatotoxins, chloroform and thioacetamide, alters the expression of hepatic xenobiotic metabolizing cytochrome P450 (CYP) enzymes of DBA/2N mice. Dose-dependent toxic effects of the two hepatotoxins were verified by histological examination. Along with the toxicity, intense staining of immunoreactive material was detected in the centrilobular zone, with anti-CYP2A5 antibody in hepatic tissue. This apparent increase in the expression of Cyp2a-5 was verified by Northern blot and Western blot analyses and by determining the enzymatic activity, coumarin 7-hydroxylase, in hepatic tissue. The results suggest that liver injury due to these hepatotoxins increases the expression of Cyp2a-5 and that the expression is pretranslationally regulated. The increased expression of Cyp2a-5 is in contrast with that of other xenobiotic metabolizing CYPs because a dose-[dose-dependent] dependent decrease of the total hepatic P450 content and either a decrease or no change in the levels of CYP1A, 2B, 2C, 2E1, and 3A4 were observed. The results suggest that essential differences exist in the regulation of CYP2A5 and other major xenobiotic metabolizing CYP enzymes and that in a damaged liver CYP2A5 may be a major catalyst of xenobiotic metabolism.en
heal.journalNameToxicol Appl Pharmacolen
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
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