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  5. Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ
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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/20116
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dc.contributor.authorPapaventsis, D.en
dc.contributor.authorSiafakas, N.en
dc.contributor.authorMarkoulatos, P.en
dc.contributor.authorPapageorgiou, G. T.en
dc.contributor.authorKourtis, C.en
dc.contributor.authorChatzichristou, E.en
dc.contributor.authorEconomou, C.en
dc.contributor.authorLevidiotou, S.en
dc.date.accessioned2015-11-24T19:04:55Z-
dc.date.available2015-11-24T19:04:55Z-
dc.identifier.issn0099-2240-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/20116-
dc.rightsDefault Licence-
dc.subject5' Untranslated Regions/geneticsen
dc.subject*Adsorptionen
dc.subjectCell Lineen
dc.subjectCollodionen
dc.subjectDNA-Binding Proteins/geneticsen
dc.subjectEnterovirus/*classification/genetics/isolation & purificationen
dc.subjectEnvironmental Monitoring/methodsen
dc.subjectHumansen
dc.subject*Micropore Filtersen
dc.subjectMolecular Sequence Dataen
dc.subjectPhylogenyen
dc.subjectPlant Proteinsen
dc.subjectPolymorphism, Restriction Fragment Lengthen
dc.subject*Reverse Transcriptase Polymerase Chain Reaction/methodsen
dc.subjectSequence Analysis, DNAen
dc.subjectSewage/*virologyen
dc.subjectTime Factorsen
dc.subjectTrans-Activatorsen
dc.subjectTranscription Factors/geneticsen
dc.subjectVirology/methodsen
dc.subject*Virus Cultivationen
dc.titleMembrane adsorption with direct cell culture combined with reverse transcription-PCR as a fast method for identifying enteroviruses from sewageen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.primary10.1128/AEM.71.1.72-79.2005-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/15640172-
heal.identifier.secondaryhttp://aem.asm.org/content/71/1/72.full.pdf-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate2005-
heal.abstractWe present a new approach for the detection and identification of enteroviruses concentrated and isolated from sewage. Samples were collected from two study sites located at Nicosia and Limassol sewage treatment plants in Cyprus. Viruses were adsorbed to cellulose nitrate membrane filters, cultured directly from the membrane filters by using the VIRADEN method, and identified by reverse transcription-PCR, followed by 5' untranslated region (5'-UTR) restriction fragment length polymorphism (RFLP) analysis and partial sequencing of the VP1 protein coding region. Initial subgrouping based on the HpaII restriction profile showed that all of the isolates except one belonged to the same genetic subcluster. Partial VP1 sequencing revealed that most isolates belonged to serotypes coxsackie B4 (42.5%) and coxsackie Alpha9 (30%), whereas coxsackie B2 (17.5%) and coxsackie B1 (3%) isolates were less frequently observed. One poliovirus type 2 isolate (2.5%) of vaccine origin was also found. The HpaII digests predicted the genetic subcluster for all isolates. They also accurately differentiated the isolates as nonpolio or polio isolates. This approach seems to be very promising for environmental surveillance of enterovirus circulation and epidemiology, with all of the significant effects that this entails for public health. Partial VP1 sequencing is efficient for molecular serotyping of enteroviruses, while 5'-UTR RFLP analysis with HpaII can also be considered an asset for the initial subclassification of enterovirus isolates.en
heal.journalNameAppl Environ Microbiolen
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
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