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  4. Τμήμα Ιατρικής
  5. Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ
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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/19849
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dc.contributor.authorTzika, A. A.en
dc.contributor.authorAstrakas, L. G.en
dc.contributor.authorCao, H.en
dc.contributor.authorMintzopoulos, D.en
dc.contributor.authorZhang, Q.en
dc.contributor.authorPadfield, K.en
dc.contributor.authorYu, H.en
dc.contributor.authorMindrinos, M. N.en
dc.contributor.authorRahme, L. G.en
dc.contributor.authorTompkins, R. G.en
dc.date.accessioned2015-11-24T19:02:55Z-
dc.date.available2015-11-24T19:02:55Z-
dc.identifier.issn1107-3756-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/19849-
dc.rightsDefault Licence-
dc.subjectAdaptor Proteins, Signal Transducing/genetics/metabolismen
dc.subjectAnimalsen
dc.subjectBiological Markers/*analysis/blooden
dc.subjectBody Surface Areaen
dc.subjectBurns/genetics/*metabolism/pathologyen
dc.subjectFatty Acids, Nonesterified/analysis/blood/metabolismen
dc.subjectGene Expression Profilingen
dc.subjectGlucose Transporter Type 4/genetics/metabolismen
dc.subjectGlycolysisen
dc.subjectHindlimb/metabolism/pathologyen
dc.subjectHumansen
dc.subjectInsulin Receptor Substrate Proteinsen
dc.subjectInsulin Resistanceen
dc.subjectLipid Metabolismen
dc.subjectLipids/*analysisen
dc.subjectMagnetic Resonance Imagingen
dc.subjectMagnetic Resonance Spectroscopyen
dc.subjectMiceen
dc.subjectMitochondria/metabolismen
dc.subjectModels, Biologicalen
dc.subjectMuscle Fibers, Skeletal/metabolism/pathologyen
dc.subjectMuscle, Skeletal/*metabolism/pathology/ultrastructureen
dc.subjectOligonucleotide Array Sequence Analysisen
dc.subjectRegression Analysisen
dc.subjectTrans-Activators/genetics/metabolismen
dc.titleMurine intramyocellular lipids quantified by NMR act as metabolic biomarkers in burn traumaen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/18506378-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate2008-
heal.abstractIt has been suggested that intramyocellular lipids (IMCLs) may serve as biomarkers of insulin resistance and mitochondrial dysfunction. Using a hind-limb mouse model of burn trauma, we tested the hypothesis that severe localized burn trauma involving 5% of the total body surface area causes a local increase in IMCLs in the leg skeletal muscle. We quantified IMCLs from ex vivo intact tissue specimens using High-Resolution Magic Angle Spinning (HRMAS) 1H NMR and characterized the accompanying gene expression patterns in burned versus control skeletal muscle specimens. We also quantified plasma-free fatty acids (FFAs) in burn versus control mice. Our results from HRMAS 1H NMR measurements indicated that IMCL levels were significantly increased in mice exposed to burn trauma. Furthermore, plasma FFA levels were also significantly increased, and gene expression of Glut4, insulin receptor substrate 1 (IRS1), glycolytic genes, and PGC-1beta was downregulated in these mice. Backward stepwise multiple linear regression analysis demonstrated that IMCL levels correlated significantly with FFA levels, which were a significant predictor of IRS1 and PGC-1beta gene expression. We conclude from these findings that IMCLs can serve as metabolic biomarkers in burn trauma and that FFAs and IMCLs may signal altered metabolic gene expression. This signaling may result in the observed burn-induced insulin resistance and skeletal muscle mitochondrial dysfunction. We believe that IMCLs may therefore be useful biomarkers in predicting the therapeutic effectiveness of hypolipidemic agents for patients with severe burns.en
heal.journalNameInt J Mol Meden
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
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