Please use this identifier to cite or link to this item:
https://olympias.lib.uoi.gr/jspui/handle/123456789/19685Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Georgatos, S. D. | en |
| dc.contributor.author | Pyrpasopoulou, A. | en |
| dc.contributor.author | Theodoropoulos, P. A. | en |
| dc.date.accessioned | 2015-11-24T19:01:24Z | - |
| dc.date.available | 2015-11-24T19:01:24Z | - |
| dc.identifier.issn | 0021-9533 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/19685 | - |
| dc.rights | Default Licence | - |
| dc.subject | Adenocarcinoma | en |
| dc.subject | Animals | en |
| dc.subject | Biological Markers | en |
| dc.subject | Endometrial Neoplasms | en |
| dc.subject | Female | en |
| dc.subject | Humans | en |
| dc.subject | Kidney/cytology | en |
| dc.subject | Lamin Type B | en |
| dc.subject | Lamins | en |
| dc.subject | Metaphase/physiology | en |
| dc.subject | Microscopy, Electron | en |
| dc.subject | Microtubules/*physiology/ultrastructure | en |
| dc.subject | Mitosis/*physiology | en |
| dc.subject | Nuclear Envelope/chemistry/*metabolism/ultrastructure | en |
| dc.subject | Nuclear Proteins/analysis | en |
| dc.subject | Prophase/physiology | en |
| dc.subject | Rats | en |
| dc.subject | Tumor Cells, Cultured | en |
| dc.title | Nuclear envelope breakdown in mammalian cells involves stepwise lamina disassembly and microtubule-drive deformation of the nuclear membrane | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/9378763 | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.publicationDate | 1997 | - |
| heal.abstract | We have studied nuclear envelope disassembly in mammalian cells by morphological methods. The first signs of nuclear lamina depolymerization become evident in early prophase as A-type lamins start dissociating from the nuclear lamina and diffuse into the nucleoplasm. While B-type lamins are still associated with the inner nuclear membrane, two symmetrical indentations develop on antidiametric sites of the nuclear envelope. These indentations accommodate the sister centrosomes and associated astral microtubules. At mid- to late prophase, elongating microtubules apparently push on the nuclear surface and eventually penetrate the nucleus. At this point the nuclear envelope becomes freely permeable to large ligands, as indicated by experiments with digitonin-treated cells and by the massive release of solubilized A-type lamins into the cytoplasm. At the prophase/prometaphase transition, the B-type lamina is fragmented, but 'islands' of lamin B polymer can still be discerned on the tips of congressing chromosomes. Finally, at metaphase, the lamin B polymer breaks down into small pieces, which tend to concentrate in the area of the mitotic spindle. Nuclear envelope breakdown is not prevented when the microtubules are depolymerized by nocodazole; however, the mode of nuclear lamina fragmentation in the absence of microtubules is markedly different from the normal one and involves multiple raffles and gaps, which develop rapidly along the entire surface of the nuclear envelope. These data suggest that nuclear envelope disassembly is a stepwise process in which the microtubules play an important part. | en |
| heal.journalName | J Cell Sci | en |
| heal.journalType | peer-reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| georgatos-1997-nuclear envelope breakdown.pdf | 4.1 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License
