1. Repository of OAI
  2. ΑΠΟΘΕΤΗΡΙΟ "ΟΛΥΜΠΙΑΣ"
  3. Σχολή Επιστημών Υγείας
  4. Τμήμα Ιατρικής
  5. Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ
Ελληνικά   English  
Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/19683
Full metadata record
DC FieldValueLanguage
dc.contributor.authorWorman, H. J.en
dc.contributor.authorLazaridis, I.en
dc.contributor.authorGeorgatos, S. D.en
dc.date.accessioned2015-11-24T19:01:23Z-
dc.date.available2015-11-24T19:01:23Z-
dc.identifier.issn0021-9258-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/19683-
dc.rightsDefault Licence-
dc.subjectAnimalsen
dc.subjectAutoantibodiesen
dc.subjectBrain Chemistryen
dc.subjectCell Nucleus/analysisen
dc.subjectElectrophoresis, Polyacrylamide Gelen
dc.subjectFemaleen
dc.subject*Gene Expression Regulationen
dc.subjectHumansen
dc.subjectImmunoassayen
dc.subjectKidney/analysisen
dc.subjectLamin Type Aen
dc.subjectLamin Type Ben
dc.subjectLaminsen
dc.subjectLiver/analysisen
dc.subjectMolecular Weighten
dc.subjectNuclear Proteins/analysis/*genetics/immunologyen
dc.subjectNucleic Acid Hybridizationen
dc.subjectPeptide Fragments/immunology/metabolismen
dc.subjectPhosphorylationen
dc.subjectRNA, Messenger/metabolismen
dc.subjectRatsen
dc.subjectRats, Inbred Strainsen
dc.subjectSpleen/analysisen
dc.subjectThiocyanatesen
dc.titleNuclear lamina heterogeneity in mammalian cells. Differential expression of the major lamins and variations in lamin B phosphorylationen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/3403563-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate1988-
heal.abstractWe have studied the molecular composition of the nuclear lamina in rat tissues of distinct embryological origin and the occurrence of the nuclear lamins during in vitro differentiation of the mouse F9 teratocarcinoma cell line. Immunochemical analysis demonstrated that all rat tissues contained the three major lamin forms (lamins A, B, and C) previously recognized in rat liver nuclei; however, other minor cross-reactive components were also identified in some tissues. The amount of the 67-kDa lamin B complexed with lamins A and C in the laminae of different tissues ranged from a stoichiometry of much less than 1 to approximately 1. Furthermore, it was found that F9 stem cells and their differentiated progeny express only lamin B, and Northern blotting analysis indicated that these cells fail to accumulate lamin A and C mRNA. Chemical cleavages and peptide mapping suggested that the 67-kDa lamin B form was of similar primary structure in all differentiated tissues and F9 cells. Employing antibodies with different affinities for phosphorylated and nonphosphorylated lamin B, we showed that the apparent invariance in the expression of this polypeptide is overriden by a heterogeneity produced via tissue-specific phosphorylation. Because similar differences in antibody recognition could be reproduced in vitro by phosphorylating lamin B with protein kinase A, we have concluded that the tissue-specific modifications of this protein may occur at consensus sites recognized by this enzyme. These data support the hypotheses that the lamins can form functional laminae by associating at various combinations, and that processes including differential lamin synthesis and post-translational modification can produce a steady state lamina heterogeneity.en
heal.journalNameJ Biol Chemen
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ

Show simple item record
Files in This Item:
There are no files associated with this item.
Show simple item record  


This item is licensed under a Creative Commons License Creative Commons