Please use this identifier to cite or link to this item:
https://olympias.lib.uoi.gr/jspui/handle/123456789/19643
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Pozidis, C. | en |
dc.contributor.author | Chalkiadaki, A. | en |
dc.contributor.author | Gomez-Serrano, A. | en |
dc.contributor.author | Stahlberg, H. | en |
dc.contributor.author | Brown, I. | en |
dc.contributor.author | Tampakaki, A. P. | en |
dc.contributor.author | Lustig, A. | en |
dc.contributor.author | Sianidis, G. | en |
dc.contributor.author | Politou, A. S. | en |
dc.contributor.author | Engel, A. | en |
dc.contributor.author | Panopoulos, N. J. | en |
dc.contributor.author | Mansfield, J. | en |
dc.contributor.author | Pugsley, A. P. | en |
dc.contributor.author | Karamanou, S. | en |
dc.contributor.author | Economou, A. | en |
dc.date.accessioned | 2015-11-24T19:01:06Z | - |
dc.date.available | 2015-11-24T19:01:06Z | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/19643 | - |
dc.rights | Default Licence | - |
dc.subject | Adenosine Triphosphatases/*chemistry/*physiology | en |
dc.subject | Amino Acid Sequence | en |
dc.subject | Bacterial Proteins/*chemistry | en |
dc.subject | Cell Membrane/enzymology/metabolism | en |
dc.subject | Chromatography | en |
dc.subject | Circular Dichroism | en |
dc.subject | Cross-Linking Reagents/pharmacology | en |
dc.subject | Detergents/pharmacology | en |
dc.subject | Dose-Response Relationship, Drug | en |
dc.subject | Escherichia coli/metabolism | en |
dc.subject | Hydrogen-Ion Concentration | en |
dc.subject | Hydrolysis | en |
dc.subject | Ions | en |
dc.subject | Kinetics | en |
dc.subject | Membrane Transport Proteins/*chemistry/*physiology | en |
dc.subject | Microscopy, Electron | en |
dc.subject | Molecular Sequence Data | en |
dc.subject | Plasmids/metabolism | en |
dc.subject | Protein Conformation | en |
dc.subject | Protein Folding | en |
dc.subject | Protein Structure, Secondary | en |
dc.subject | Protein Structure, Tertiary | en |
dc.subject | Protein Transport | en |
dc.subject | Pseudomonas/*enzymology | en |
dc.subject | Subcellular Fractions | en |
dc.subject | Temperature | en |
dc.subject | Water/metabolism | en |
dc.title | Type III protein translocase: HrcN is a peripheral ATPase that is activated by oligomerization | en |
heal.type | journalArticle | - |
heal.type.en | Journal article | en |
heal.type.el | Άρθρο Περιοδικού | el |
heal.identifier.primary | 10.1074/jbc.M301903200 | - |
heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/12734178 | - |
heal.identifier.secondary | http://www.jbc.org/content/278/28/25816.full.pdf | - |
heal.language | en | - |
heal.access | campus | - |
heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
heal.publicationDate | 2003 | - |
heal.abstract | Type III protein secretion (TTS) is catalyzed by translocases that span both membranes of Gram-negative bacteria. A hydrophilic TTS component homologous to F1/V1-ATPases is ubiquitous and essential for secretion. We show that hrcN encodes the putative TTS ATPase of Pseudomonas syringae pathovar phaseolicola and that HrcN is a peripheral protein that assembles in clusters at the membrane. A decahistidinyl HrcN derivative was overexpressed in Escherichia coli and purified to homogeneity in a folded state. Hydrodynamic analysis, cross-linking, and electron microscopy revealed four distinct HrcN forms: I, 48 kDa (monomer); II, approximately 300 kDa (putative hexamer); III, 575 kDa (dodecamer); and IV, approximately 3.5 MDa. Form III is the predominant form of HrcN at the membrane, and its ATPase activity is dramatically stimulated (>700-fold) over the basal activity of Form I. We propose that TTS ATPases catalyze protein translocation as activated homo-oligomers at the plasma membrane. | en |
heal.journalName | J Biol Chem | en |
heal.journalType | peer-reviewed | - |
heal.fullTextAvailability | TRUE | - |
Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Pozidis-2003-Type III protein tra.pdf | 612.73 kB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License