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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Munz, C. | en |
| dc.contributor.author | Hofmann, M. | en |
| dc.contributor.author | Yoshida, K. | en |
| dc.contributor.author | Moustakas, A. K. | en |
| dc.contributor.author | Kikutani, H. | en |
| dc.contributor.author | Stevanovic, S. | en |
| dc.contributor.author | Papadopoulos, G. K. | en |
| dc.contributor.author | Rammensee, H. G. | en |
| dc.date.accessioned | 2015-11-24T18:59:46Z | - |
| dc.date.available | 2015-11-24T18:59:46Z | - |
| dc.identifier.issn | 0014-2980 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/19406 | - |
| dc.rights | Default Licence | - |
| dc.subject | Amino Acid Motifs | en |
| dc.subject | Amino Acid Sequence | en |
| dc.subject | Animals | en |
| dc.subject | Cell Line | en |
| dc.subject | Diabetes Mellitus, Type 1/genetics/*immunology | en |
| dc.subject | Histocompatibility Antigens Class II/analysis/*chemistry | en |
| dc.subject | Mice | en |
| dc.subject | Mice, Inbred NOD | en |
| dc.subject | Models, Structural | en |
| dc.subject | Molecular Sequence Data | en |
| dc.title | Peptide analysis, stability studies, and structural modeling explain contradictory peptide motifs and unique properties of the NOD mouse MHC class II molecule H2-A(g7) | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.primary | 10.1002/1521-4141(200208)32:8<2105::AID-IMMU2105>3.0.CO;2-Q | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/12209622 | - |
| heal.identifier.secondary | http://onlinelibrary.wiley.com/store/10.1002/1521-4141(200208)32:8<2105::AID-IMMU2105>3.0.CO;2-Q/asset/2105_ftp.pdf?v=1&t=h0ustzd0&s=7a6c29c79d6fe9373d459190278fbbf9a64d24ff | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.publicationDate | 2002 | - |
| heal.abstract | The MHC class II molecule H2-A(g7) is the chief genetic determinant in insulin-dependent diabetes mellitus of the non-obese diabetic (NOD) mice. Poor peptide binding ability, as well as presentation of a unique subset of peptides by this molecule was suggested to promote autoimmunity in this strain. However, several laboratories have presented results in favor of an H2-A(g7) molecule that can avidly bind many different peptides. The crystal structures of H2-A(g7) in complex with two different peptides did not completely resolve this issue. To analyze the peptide binding capacity and the motif requirements of H2-A(g7), we eluted natural ligands from purified H2-A(g7) molecules isolated from the H2-A(g7)-transfected M12-C3 cells. A low peptide yield dominated by a few peptide ligands was found. Pool sequencing and alignment of individual ligands on the basis of molecular modeling revealed a peptide-binding motif with basic/aliphatic/small hydrophilic amino acids at relative position 1 (p1), aliphatic amino acids at p4, Ala at p6, and acidic amino acids and Ser/Gly at p9, as well as acidic residues at p10/11. Though weak, the binding of individual ligands, as well as the importance of an acidic C-terminal residue was confirmed by peptide binding studies to isolated H2-A(g7) molecules. Furthermore, the H2-A(g7) molecule incompletely dissociated into its constituent chains in SDS-electrophoresis under nonreducing conditions. This provides additional evidence of its weak affinity for peptides, which probably arises from the combination of beta56His/beta57Ser/beta78Ala and other unique H2-A(g7) residues in contact with the antigenic peptide. These results allow a better understanding of the role of this molecule in the development of autoimmunity and the identification of epitopes relevant to diabetes. | en |
| heal.journalName | Eur J Immunol | en |
| heal.journalType | peer-reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Munz-2002-Peptide analysis, st.pdf | 328.43 kB | Adobe PDF | View/Open Request a copy |
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