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  5. Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ
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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/18934
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dc.contributor.authorAstrakas, L. G.en
dc.contributor.authorGoljer, I.en
dc.contributor.authorYasuhara, S.en
dc.contributor.authorPadfield, K. E.en
dc.contributor.authorZhang, Q.en
dc.contributor.authorGopalan, S.en
dc.contributor.authorMindrinos, M. N.en
dc.contributor.authorDai, G.en
dc.contributor.authorYu, Y. M.en
dc.contributor.authorMartyn, J. A.en
dc.contributor.authorTompkins, R. G.en
dc.contributor.authorRahme, L. G.en
dc.contributor.authorTzika, A. A.en
dc.date.accessioned2015-11-24T18:55:49Z-
dc.date.available2015-11-24T18:55:49Z-
dc.identifier.issn1530-6860-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/18934-
dc.rightsDefault Licence-
dc.subject*Apoptosisen
dc.subjectBurns/*metabolism/pathologyen
dc.subjectFatty Acids/metabolismen
dc.subjectGene Expression Profilingen
dc.subjectIn Situ Nick-End Labelingen
dc.subject*Lipid Metabolismen
dc.subjectMagnetic Resonance Spectroscopyen
dc.subjectMitochondria/physiologyen
dc.subjectMuscle, Skeletal/*metabolismen
dc.subjectOligonucleotide Array Sequence Analysisen
dc.subjectOxidation-Reductionen
dc.titleProton NMR spectroscopy shows lipids accumulate in skeletal muscle in response to burn trauma-induced apoptosisen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.primary10.1096/fj.04-2005com-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/16126910-
heal.identifier.secondaryhttp://www.fasebj.org/content/19/11/1431.full.pdf-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate2005-
heal.abstractBurn trauma triggers hypermetabolism and muscle wasting via increased cellular protein degradation and apoptosis. Proton nuclear magnetic resonance (1H NMR) spectroscopy can detect mobile lipids in vivo. To examine the local effects of burn in skeletal muscle, we performed in vivo 1H NMR on mice 3 days after burn trauma; and ex vivo, high-resolution, magic angle spinning (1)H NMR on intact excised mouse muscle samples before and 1 and 3 days after burn. These samples were then analyzed for apoptotic nuclei using a terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay. To confirm our NMR and cell biology results, we used transcriptome analysis to demonstrate that burn trauma alters the expression of genes involved in lipid metabolism and apoptosis. Our results demonstrate that burn injury results in a localized intramyocellular lipid accumulation, which in turn is accompanied by burn-induced apoptosis and mitochondrial dysfunction, as seen by the up-regulation of apoptotic genes and down-regulation of genes that encode lipid oxidation and the peroxisomal proliferator activator receptor gamma coactivator PGC-1beta. Moreover, the increased levels of bisallylic methylene fatty acyl protons (2.8 ppm) and vinyl protons (5.4 ppm), in conjunction with the TUNEL assay results, further suggest that burn trauma results in apoptosis. Together, our results provide new insight into the local physiological changes that occur in skeletal muscle after severe burn trauma.en
heal.journalNameFaseb Journalen
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
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