1. Repository of OAI
  2. ΑΠΟΘΕΤΗΡΙΟ "ΟΛΥΜΠΙΑΣ"
  3. Σχολή Επιστημών Υγείας
  4. Τμήμα Ιατρικής
  5. Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ
Ελληνικά   English  
Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/18793
Full metadata record
DC FieldValueLanguage
dc.contributor.authorKesanopoulos, K.en
dc.contributor.authorTzanakaki, G.en
dc.contributor.authorVelegraki, A.en
dc.contributor.authorTegos, N.en
dc.contributor.authorCaugant, D. A.en
dc.contributor.authorMenounos, P.en
dc.contributor.authorKourea-Kremastinou, J.en
dc.contributor.authorLevidiotou-Stefanou, S.en
dc.date.accessioned2015-11-24T18:55:02Z-
dc.date.available2015-11-24T18:55:02Z-
dc.identifier.issn0928-8244-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/18793-
dc.rightsDefault Licence-
dc.subjectBacterial Typing Techniquesen
dc.subjectBase Sequenceen
dc.subjectChilden
dc.subjectDNA, Bacterial/geneticsen
dc.subjectDisease Outbreaksen
dc.subjectGenes, Bacterialen
dc.subjectGreece/epidemiologyen
dc.subjectHumansen
dc.subjectMeningococcal Infections/epidemiology/microbiologyen
dc.subjectMolecular Epidemiologyen
dc.subjectNeisseria meningitidis/*classification/*genetics/isolation & purificationen
dc.subjectPhenotypeen
dc.subjectPolymerase Chain Reaction/*methodsen
dc.subject*Polymorphism, Single-Stranded Conformationalen
dc.subjectPorins/geneticsen
dc.subjectSerotypingen
dc.titleRapid molecular identification of Neisseria meningitidis isolates using the polymerase chain reaction followed by single-stranded conformation polymorphism analysisen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.primary10.1016/j.femsim.2005.03.003-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/16051066-
heal.identifier.secondaryhttp://onlinelibrary.wiley.com/store/10.1016/j.femsim.2005.03.003/asset/j.femsim.2005.03.003.pdf?v=1&t=h0aj42he&s=9b3062d7f4bdac86601c1eb2371dfdd4ee428abb-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate2005-
heal.abstractTyping of Neisseria meningitidis strains is currently performed with conventional and molecular methods. Our objectives were: first, to develop a polymerase chain reaction (PCR) followed by single-stranded conformation polymorphism (SSCP) analysis of the PorA gene (VR1 region) to distinguish N. meningitidis subtypes and second, to evaluate the method for the identification and characterization of N. meningitidis in patient specimens. SSCP analysis of the VR1 region of the PorA1/2 gene from 126 N. meningitidis strains and 29 clinical samples identified seven SSCP types (SP-1 to SP-7); four strains were not typeable by the method. Classification according to the SSCP methods and serosubtype agreed for 122 of the 126 typeable strains (96.8%). For the 24-culture positive clinical samples, serosubtype and SSCP agreed in all cases. Five samples, which were culture-negative but obtained from children during an apparent outbreak of meningococcal disease in a primary school, presented identical SSCP classification for each sample (SP-2). PCR-SSCP is a rapid and cost-effective method for typing N. meningitidis strains that could provide important early information in the surveillance of suspected meningococcal outbreaks, particularly when culture-negative specimens constitutes the main source of material to analyze.en
heal.journalNameFEMS Immunol Med Microbiolen
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ

Show simple item record
Files in This Item:
File Description SizeFormat 
Kesanopoulos-2005-Rapid molecular iden.pdf241.74 kBAdobe PDFView/Open    Request a copy
Show simple item record  


This item is licensed under a Creative Commons License Creative Commons