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  5. Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ
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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/18735
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dc.contributor.authorWei, Q.en
dc.contributor.authorBondy, M. L.en
dc.contributor.authorMao, L.en
dc.contributor.authorGaun, Y.en
dc.contributor.authorCheng, L.en
dc.contributor.authorCunningham, J.en
dc.contributor.authorFan, Y.en
dc.contributor.authorBruner, J. M.en
dc.contributor.authorYung, W. K.en
dc.contributor.authorLevin, V. A.en
dc.contributor.authorKyritsis, A. P.en
dc.date.accessioned2015-11-24T18:54:45Z-
dc.date.available2015-11-24T18:54:45Z-
dc.identifier.issn0008-5472-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/18735-
dc.rightsDefault Licence-
dc.subjectAdaptor Proteins, Signal Transducingen
dc.subject*Adenosine Triphosphatasesen
dc.subject*Carrier Proteinsen
dc.subject*DNA Repairen
dc.subject*DNA Repair Enzymesen
dc.subjectDNA-Binding Proteins/*geneticsen
dc.subjectFungal Proteins/*geneticsen
dc.subjectGene Expression Regulation, Neoplasticen
dc.subjectGlioma/*geneticsen
dc.subjectHumansen
dc.subjectMicrosatellite Repeatsen
dc.subjectMutS Homolog 2 Proteinen
dc.subject*Neoplasm Proteinsen
dc.subjectPolymerase Chain Reaction/methodsen
dc.subjectProteins/*geneticsen
dc.subjectRNA, Messenger/geneticsen
dc.subjectRNA, Neoplasm/geneticsen
dc.subjectSaccharomyces cerevisiae Proteinsen
dc.titleReduced expression of mismatch repair genes measured by multiplex reverse transcription-polymerase chain reaction in human gliomasen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/9135006-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate1997-
heal.abstractMicrosatellite instability (MIN) is frequently observed in hereditary nonpolyposis colon cancer and in other sporadic cancers including gliomas. Abnormalities in at least one of five mismatch repair (MMR) genes are implicated in the development of cancers in hereditary nonpolyposis colon cancer and the associated MIN. Using a newly developed multiplex reverse transcription-PCR assay, we evaluated the expression of the five known human MMR genes (hMSH2, hMLH1, hPMS1, hPMS2, and GTBP) in human gliomas by measuring simultaneously the relative levels of the transcripts. The beta-actin gene was used as an internal control for RNA degradation and DNA contamination and as a reference for quantifying the levels of their transcripts. Of the 33 gliomas examined, 42% (14) had low expression of hMSH2 (at least 4-5-fold lower than normal mean), 21% (7) had low expression of hMLH1, and 18% (6) had low expression of hPMS1 compared with the expression in the lymphocytes from 13 normal individuals. Furthermore, six of the 33 (18%) tumor samples had decreased expression of more than one MMR gene. Two of these six patients with multiple gene abnormalities had second primary cancers, and an additional patient had multifocal gliomas. Further molecular analysis of available DNA samples indicated that one of five of those tumors with aberrant expression of MMR genes had MIN, as compared with none of five tumors with normal expression. These data suggest that reduced expression of MMR genes is frequent in human gliomas and that aberrant expression of more than one MMR gene may be associated with increased risk of second primary malignancies in glioma patients.en
heal.journalNameCancer Resen
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
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