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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Buffinton, G. | en |
| dc.contributor.author | Mira, D. | en |
| dc.contributor.author | Galaris, D. | en |
| dc.contributor.author | Hochstein, P. | en |
| dc.contributor.author | Cadenas, E. | en |
| dc.date.accessioned | 2015-11-24T18:54:43Z | - |
| dc.date.available | 2015-11-24T18:54:43Z | - |
| dc.identifier.issn | 0009-2797 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/18729 | - |
| dc.rights | Default Licence | - |
| dc.subject | Chemical Phenomena | en |
| dc.subject | Chemistry | en |
| dc.subject | *Ferrous Compounds | en |
| dc.subject | *Glutathione | en |
| dc.subject | *Hemeproteins | en |
| dc.subject | Kinetics | en |
| dc.subject | *Metmyoglobin | en |
| dc.subject | *Myoglobin/analogs & derivatives | en |
| dc.subject | Oxidation-Reduction | en |
| dc.subject | Oxygen/*pharmacology | en |
| dc.subject | Spectrophotometry | en |
| dc.subject | *Vitamin K | en |
| dc.title | Reduction of ferryl- and metmyoglobin to ferrous myoglobin by menadione-glutathione conjugate. Spectrophotometric studies under aerobic and anaerobic conditions | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/3396121 | - |
| heal.identifier.secondary | http://www.sciencedirect.com/science/article/pii/0009279788900725 | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.publicationDate | 1988 | - |
| heal.abstract | Both metmyoglobin (MbIII) and ferrylmyoglobin (MbIV) are reduced by the menadiol-glutathione conjugate (GS-Q2-) to oxymyoglobin (MbIIO2) or deoxymyoglobin (MbII), depending whether the assay is carried out under aerobic or anaerobic conditions, respectively. Under aerobic conditions, the reduction of MbIII to MbIIO2 by GS-Q2- is associated with O2 consumption. The latter process is accounted for by (a) the autoxidation of the conjugate yielding H2O2 and (b) the rapid binding of O2 to MbII to yield MbIIO2. The ratio [O2]consumed/[MbIIO2]formed is approximately 1.5 at the time when MbIIO2 formation is maximal (at about 0.8 min). This ratio, higher than the unit, indicates that there is more than one O2-consuming reaction in this experimental model. The ratio of initial rates of O2 consumption and MbIIO2 formation is close to the unit [(-dO2/dt)/(+ dMbIIO2/dt) = 1.1]. The formation of H2O2 originating during the autoxidation of the GS-Q2- is substantially lower in the presence of MbIII, probably due to the heterolytic cleavage of the O--O bond of the peroxide by the hemoprotein. Although the latter reaction should yield MbIV, this species is not observed in the absorption spectrum, probably due to its rapid reduction by GS-Q2-. MbIV is reduced to MbIIO2 by the GS-Q2-. Whether this reaction takes place in one-electron transfer steps, that is, the sequence: MbIV----MbIII----MbIIO2 is difficult to evaluate by absorption spectral analysis, due to the rapid rate of the [MbIV----MbIIO2] transition. Under anaerobic conditions, the reduction of either MbIII or MbIV by GS-Q2- yields MbII as a stable molecular product. Anaerobic conditions prevent any further interaction of MbII with intermediates of O2 reduction derived from GS-Q2- autoxidation. | en |
| heal.journalName | Chem Biol Interact | en |
| heal.journalType | peer-reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Galaris-1988-reduction of ferryl.pdf | 931.57 kB | Adobe PDF | View/Open Request a copy |
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