Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/18588
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dc.contributor.authorHeikkinen, R.en
dc.contributor.authorFotsis, T.en
dc.contributor.authorAdlercreutz, H.en
dc.date.accessioned2015-11-24T18:53:39Z-
dc.date.available2015-11-24T18:53:39Z-
dc.identifier.issn0009-9147-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/18588-
dc.rightsDefault Licence-
dc.subjectChromatography, High Pressure Liquid/methodsen
dc.subjectEstradiol Congenersen
dc.subjectEstrogens/blood/*isolation & purification/urineen
dc.subjectFemaleen
dc.subjectHumansen
dc.subjectIndicators and Reagentsen
dc.subjectMaleen
dc.subjectPregnancyen
dc.subjectSex Factorsen
dc.titleReversed-phase C18 cartridge for extraction of estrogens from urine and plasmaen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/7237782-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate1981-
heal.abstractWe describe a simple method for extraction of estrogens that is efficient over the whole polarity range of estrogen metabolites in urine and plasma. A small reversed-phase C18 cartridge (Sep-Pak C18, Waters Associates, Inc.) is used for this purpose. After application of the biological fluid, followed by an aqueous wash, estrogens are eluted in 3 mL of methanol. For urine the pH should be kept at 3 before the methanol elution. When estrogens are extracted from plasma, the steroid-protein interaction is counteracted by the presence of triethylammonium sulfate and D-norgestrel at pH 5. The recoveries of added 3H-labeled estrogens are essentially quantitative. The capacity of the column is high and estrogens can be extracted from large volumes of pregnancy urine without loss in the washing water. Because of the high flow rate (2 mL/min) and the small volume of methanol in which the estrogens are eluted, the whole procedure is convenient and rapid.en
heal.journalNameClin Chemen
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ

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