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https://olympias.lib.uoi.gr/jspui/handle/123456789/18551Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | El-Zein, R. | en |
| dc.contributor.author | Bondy, M. L. | en |
| dc.contributor.author | Wang, L. E. | en |
| dc.contributor.author | de Andrade, M. | en |
| dc.contributor.author | Sigurdson, A. J. | en |
| dc.contributor.author | Bruner, J. M. | en |
| dc.contributor.author | Kyritsis, A. P. | en |
| dc.contributor.author | Levin, V. A. | en |
| dc.contributor.author | Wei, Q. | en |
| dc.date.accessioned | 2015-11-24T18:53:23Z | - |
| dc.date.available | 2015-11-24T18:53:23Z | - |
| dc.identifier.issn | 0027-5107 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/18551 | - |
| dc.rights | Default Licence | - |
| dc.subject | Adult | en |
| dc.subject | Central Nervous System Neoplasms/*genetics/pathology | en |
| dc.subject | Chromosome Aberrations | en |
| dc.subject | Female | en |
| dc.subject | Genetic Testing/*methods | en |
| dc.subject | Glioma/*genetics/pathology | en |
| dc.subject | Humans | en |
| dc.subject | In Situ Hybridization, Fluorescence/*methods | en |
| dc.subject | Male | en |
| dc.subject | Micronucleus Tests/*methods | en |
| dc.subject | Middle Aged | en |
| dc.title | Risk assessment for developing gliomas: a comparison of two cytogenetic approaches | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/11152970 | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.publicationDate | 2001 | - |
| heal.abstract | Chromosome instability (CIN) measured as chromosome aberrations has long been suggested as a cancer susceptibility biomarker. Conventional cytogenetic end-points are now being improved by combining molecular methods, which increases the sensitivity, specificity, and precision of the assay. In this study we examined both spontaneous and gamma-ray induced CIN in lymphocyte cultures from 51 previously untreated glioma patients and 51 age-, sex- and ethnicity-matched controls. CIN was assessed using two parallel methods: (1) the mutagen sensitivity (MS) assay and (2) the multicolor fluorescence in situ hybridization (FISH) assay. The frequency of spontaneous breaks was significantly higher in glioma patients (mean+/-S.D., 2.12+/-1.07) than in controls (1.24+/-0.86, P<0.001) when using the FISH assay but not the MS assay (0.019+/-0.02 and 0.019+/-0.01, respectively; P=0.915). Similarly, the frequency of induced chromatid breaks was significantly higher using the FISH assay (3.39+/-1.72) but not the MS assay (0.42+/-0.16) in the patients versus controls (2.08+/-1.18 and 0.37+/-0.15, respectively; P<0.001 and P=0.10, respectively). By using the median number of breaks in the controls as the cutoff value, we observed an odds ratio (ORs) of 5.13 (95% CI=2.23-12.1) for spontaneous and 4.86 (95% CI=2.08-11.4) for induced CIN using the FISH assay, whereas the ORs were 1.32 (95% CI=0.49-3.58) and 1.28 (95% CI=0.59-2.80) for spontaneous and induced CIN using the MS assay. There was also a significant increase in the frequency of hyperdiploid cells in the glioma cases which could only be detected using the FISH assay (OR=4.0, 95% CL=0.9-17.0). By combining both methods an estimated risk of 7.0 (95% CI=1.7-25.6) was observed. There was no correlation between the breaks detected by the two methods suggesting that each method is a measure of a different event. The results indicate that using the multicolor FISH assay for detection of CIN in peripheral blood lymphocytes in glioma patients is a more useful marker for risk assessment. | en |
| heal.journalName | Mutat Res | en |
| heal.journalType | peer-reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ | |
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