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dc.contributor.authorEl-Zein, R.en
dc.contributor.authorBondy, M. L.en
dc.contributor.authorWang, L. E.en
dc.contributor.authorde Andrade, M.en
dc.contributor.authorSigurdson, A. J.en
dc.contributor.authorBruner, J. M.en
dc.contributor.authorKyritsis, A. P.en
dc.contributor.authorLevin, V. A.en
dc.contributor.authorWei, Q.en
dc.rightsDefault Licence-
dc.subjectCentral Nervous System Neoplasms/*genetics/pathologyen
dc.subjectChromosome Aberrationsen
dc.subjectGenetic Testing/*methodsen
dc.subjectIn Situ Hybridization, Fluorescence/*methodsen
dc.subjectMicronucleus Tests/*methodsen
dc.subjectMiddle Ageden
dc.titleRisk assessment for developing gliomas: a comparison of two cytogenetic approachesen
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.abstractChromosome instability (CIN) measured as chromosome aberrations has long been suggested as a cancer susceptibility biomarker. Conventional cytogenetic end-points are now being improved by combining molecular methods, which increases the sensitivity, specificity, and precision of the assay. In this study we examined both spontaneous and gamma-ray induced CIN in lymphocyte cultures from 51 previously untreated glioma patients and 51 age-, sex- and ethnicity-matched controls. CIN was assessed using two parallel methods: (1) the mutagen sensitivity (MS) assay and (2) the multicolor fluorescence in situ hybridization (FISH) assay. The frequency of spontaneous breaks was significantly higher in glioma patients (mean+/-S.D., 2.12+/-1.07) than in controls (1.24+/-0.86, P<0.001) when using the FISH assay but not the MS assay (0.019+/-0.02 and 0.019+/-0.01, respectively; P=0.915). Similarly, the frequency of induced chromatid breaks was significantly higher using the FISH assay (3.39+/-1.72) but not the MS assay (0.42+/-0.16) in the patients versus controls (2.08+/-1.18 and 0.37+/-0.15, respectively; P<0.001 and P=0.10, respectively). By using the median number of breaks in the controls as the cutoff value, we observed an odds ratio (ORs) of 5.13 (95% CI=2.23-12.1) for spontaneous and 4.86 (95% CI=2.08-11.4) for induced CIN using the FISH assay, whereas the ORs were 1.32 (95% CI=0.49-3.58) and 1.28 (95% CI=0.59-2.80) for spontaneous and induced CIN using the MS assay. There was also a significant increase in the frequency of hyperdiploid cells in the glioma cases which could only be detected using the FISH assay (OR=4.0, 95% CL=0.9-17.0). By combining both methods an estimated risk of 7.0 (95% CI=1.7-25.6) was observed. There was no correlation between the breaks detected by the two methods suggesting that each method is a measure of a different event. The results indicate that using the multicolor FISH assay for detection of CIN in peripheral blood lymphocytes in glioma patients is a more useful marker for risk assessment.en
heal.journalNameMutat Resen
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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