Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/18411
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dc.contributor.authorKondraganti, S.en
dc.contributor.authorMohanam, S.en
dc.contributor.authorChintala, S. K.en
dc.contributor.authorKin, Y.en
dc.contributor.authorJasti, S. L.en
dc.contributor.authorNirmala, C.en
dc.contributor.authorLakka, S. S.en
dc.contributor.authorAdachi, Y.en
dc.contributor.authorKyritsis, A. P.en
dc.contributor.authorAli-Osman, F.en
dc.contributor.authorSawaya, R.en
dc.contributor.authorFuller, G. N.en
dc.contributor.authorRao, J. S.en
dc.date.accessioned2015-11-24T18:52:32Z-
dc.date.available2015-11-24T18:52:32Z-
dc.identifier.issn0008-5472-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/18411-
dc.rightsDefault Licence-
dc.subjectAnimalsen
dc.subjectBlotting, Northernen
dc.subjectBlotting, Westernen
dc.subjectBrain/metabolismen
dc.subjectCell Lineen
dc.subjectCells, Cultureden
dc.subjectCoculture Techniquesen
dc.subjectCollagen/metabolismen
dc.subjectDNA, Complementary/metabolismen
dc.subjectDown-Regulationen
dc.subjectDrug Combinationsen
dc.subject*Gene Transfer Techniquesen
dc.subjectGlioblastoma/*drug therapy/*enzymology/*geneticsen
dc.subjectHumansen
dc.subjectLaminin/metabolismen
dc.subjectMatrix Metalloproteinase 9/*metabolismen
dc.subjectMiceen
dc.subjectMice, Nudeen
dc.subjectMicroscopy, Confocalen
dc.subjectNeoplasm Invasivenessen
dc.subjectNeoplasm Transplantationen
dc.subjectOligonucleotides/pharmacologyen
dc.subjectOligonucleotides, Antisense/*pharmacologyen
dc.subjectPlasmids/metabolismen
dc.subjectProteoglycans/metabolismen
dc.subjectRNA, Messenger/metabolismen
dc.subjectRatsen
dc.subjectTime Factorsen
dc.subjectTransfectionen
dc.titleSelective suppression of matrix metalloproteinase-9 in human glioblastoma cells by antisense gene transfer impairs glioblastoma cell invasionen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/11156378-
heal.identifier.secondaryhttp://cancerres.aacrjournals.org/content/60/24/6851.full.pdf-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate2000-
heal.abstractIncreased expression of matrix metalloproteinases (MMPs) has been associated with human glioblastoma tumor progression. In this study, we sought to down-regulate MMP-9 expression by stably transfecting a high-grade glioblastoma cell line with a plasmid vector capable of expressing an antisense transcript complementary to a 528-bp segment at the 5' end of human MMP-9 cDNA. Stable transfectants were obtained through selection with G418. Of the clones transfected with vector, sense, and antisense constructs, Northern blotting, Western blotting, and gelatin zymography showed that MMP-9 expression was significantly reduced only in the antisense-transfected cells. A Matrigel invasion assay revealed marked reductions in invasiveness for the antisense clones relative to the parental, vector, and sense clones. Cocultures of tumor spheroids and fetal rat brain aggregates showed that the antisense-transfected stable clones showed no invasion of the rat brain aggregates; in contrast, 90% of the parental, vector, and sense clones invaded the rat brain aggregates. Intracerebral injection of antisense stable transfectants in nude mice produced no tumors or very small tumors, but intracerebral injection of parental or vector clones did produce tumors. These results suggest that MMP-9 expression is essential for the invasiveness of glioblastoma cells.en
heal.journalNameCancer Resen
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ

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