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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/18264
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dc.contributor.authorDoulias, P. T.en
dc.contributor.authorBarbouti, A.en
dc.contributor.authorGalaris, D.en
dc.contributor.authorIschiropoulos, H.en
dc.date.accessioned2015-11-24T18:51:27Z-
dc.date.available2015-11-24T18:51:27Z-
dc.identifier.issn0891-5849-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/18264-
dc.rightsDefault Licence-
dc.subjectAscorbic Acid/analogs & derivatives/pharmacologyen
dc.subjectCalcium/metabolismen
dc.subjectCatalase/metabolismen
dc.subjectChelating Agents/pharmacologyen
dc.subject*Comet Assayen
dc.subjectDNA Damage/*drug effectsen
dc.subjectDNA, Single-Stranded/drug effects/genetics/metabolismen
dc.subjectData Interpretation, Statisticalen
dc.subjectDose-Response Relationship, Drugen
dc.subjectEgtazic Acid/analogs & derivatives/pharmacologyen
dc.subjectFlow Cytometryen
dc.subjectHumansen
dc.subjectKineticsen
dc.subjectLymphocytes/*drug effects/metabolismen
dc.subjectMicroscopy, Ultravioleten
dc.subjectMolsidomine/analogs & derivatives/antagonists & inhibitors/*pharmacologyen
dc.subjectNitrates/metabolismen
dc.subjectNitric Oxide Donors/antagonists & inhibitors/*pharmacologyen
dc.subjectSuperoxide Dismutase/metabolismen
dc.titleSIN-1-induced DNA damage in isolated human peripheral blood lymphocytes as assessed by single cell gel electrophoresis (comet assay)en
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/11295366-
heal.identifier.secondaryhttp://www.sciencedirect.com/science/article/pii/S0891584900005116-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate2001-
heal.abstractHuman lymphocytes were exposed to increasing concentrations of SIN-1, which generates superoxide and nitric oxide, and the formation of single-strand breaks (SSB) in individual cells was determined by the single-cell gel electrophoresis assay (comet assay). A dose- and time-dependent increase in SSB formation was observed rapidly after the addition of SIN-1 (0.1-15 mM). Exposure of the cells to SIN-1 (5 mM) in the presence of excess of superoxide dismutase (0.375 mM) increased the formation of SSB significantly, whereas 1000 U/ml catalase significantly decreased the quantity of SSB. The simultaneous presence of both superoxide dismutase and catalase before the addition of SIN-1 brought the level of SSB to that of the untreated cells. Moreover, pretreatment of the cells with the intracellular Ca(2+)-chelator BAPTA/AM inhibited SIN-1-induced DNA damage, indicating the involvement of intracellular Ca(2+) changes in this process. On the other hand, pretreatment of the same cells with ascorbate or dehydroascorbate did not offer any significant protection in this system. The data suggest that H2O2-induced changes in Ca(2+) homeostasis are the predominant pathway for the induction of SSB in human lymphocytes exposed to oxidants.en
heal.journalNameFree Radic Biol Meden
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
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