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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Antila, E. | en |
| dc.contributor.author | Fotsis, T. | en |
| dc.contributor.author | Wartiovaara, J. | en |
| dc.contributor.author | Adlercreutz, H. | en |
| dc.date.accessioned | 2015-11-24T18:50:33Z | - |
| dc.date.available | 2015-11-24T18:50:33Z | - |
| dc.identifier.issn | 0022-4731 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/18097 | - |
| dc.rights | Default Licence | - |
| dc.subject | Cell Line | en |
| dc.subject | Chromatography, Thin Layer | en |
| dc.subject | Female | en |
| dc.subject | Gas Chromatography-Mass Spectrometry | en |
| dc.subject | Humans | en |
| dc.subject | Kinetics | en |
| dc.subject | Ovarian Neoplasms/*metabolism | en |
| dc.subject | Sex Hormone-Binding Globulin/metabolism | en |
| dc.subject | Steroids/isolation & purification/*metabolism | en |
| dc.subject | Teratoma/*metabolism | en |
| dc.title | Steroid metabolism in human teratocarcinoma cell line PA 1 | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/6685797 | - |
| heal.identifier.secondary | http://ac.els-cdn.com/0022473183903746/1-s2.0-0022473183903746-main.pdf?_tid=f1d4b1123b4116b035c8bdf74407f2c1&acdnat=1332750986_06ab269c5bc5b75d32cad8e63b5e8e97 | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.publicationDate | 1983 | - |
| heal.abstract | Human ovarian teratocarcinoma cells of line PA 1, (Zeuthen et al., 1979[1]) used as model for early embryonic cells, were analyzed for their in vitro capacity to convert steroids. The cells were incubated for 20 h with radioactive pregnenolone, progesterone, dehydroepiandrosterone, androstenedione, testosterone or estradiol-17 beta, or with non-radioactive progesterone, 6 alpha- or 6 beta-hydroxyprogesterone, 3 beta-hydroxy-5 alpha-pregnan-20-one, dehydroepiandrosterone or estradiol-17 beta. The metabolites were analyzed by thin layer chromatography or studied by gas chromatography-mass spectrometry. The results indicate that PA 1 cells are able to metabolize, although to a restricted amount, a variety of steroids, most markedly progesterone. The metabolites were almost exclusively found in the medium. The main metabolite of progesterone was 3 beta, 6 alpha-dihydroxy-5 alpha-pregnan-20-one. Minor formation of progesterone from pregnenolone could be detected. Human chorionic gonadotropin did not have any effect on pregnenolone metabolism. No formation of estradiol-17 beta or estrone from dehydroepiandrosterone, androstenedione or testosterone could be detected. However, estradiol-17 beta was shown to be converted mainly to estrone. These findings indicate that undifferentiated PA 1 teratocarcinoma cells like certain mouse teratocarcinoma cells, seem not to be steroidogenic but are capable of metabolizing naturally occurring steroid hormones and their precursors. | en |
| heal.journalName | J Steroid Biochem | en |
| heal.journalType | peer-reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Antila-1983-Steroid metabolism i.pdf | 659.82 kB | Adobe PDF | View/Open Request a copy |
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