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  5. Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά). ΧΗΜ
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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/10650
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dc.contributor.authorBairaktari, E.en
dc.contributor.authorElisaf, M.en
dc.contributor.authorTzallas, C.en
dc.contributor.authorKarabina, S. A.en
dc.contributor.authorTselepis, A. D.en
dc.contributor.authorSiamopoulos, K. C.en
dc.contributor.authorTsolas, O.en
dc.date.accessioned2015-11-24T16:58:04Z-
dc.date.available2015-11-24T16:58:04Z-
dc.identifier.issn0009-9120-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/10650-
dc.rightsDefault Licence-
dc.subjectmethod comparisonen
dc.subjecttriglyceridesen
dc.subjecthemodialysis patientsen
dc.subjectchronic-renal-failureen
dc.subjectdextran sulfate-mg2+ methoden
dc.subjecthdl-cholesterolen
dc.subjectlipid abnormalitiesen
dc.subjecthomogeneous assayen
dc.subjectcardiovascular-diseaseen
dc.subjectanalytical performanceen
dc.subjectmulticenter evaluationen
dc.subjectpediatric populationen
dc.subjectclinical-performanceen
dc.titleEvaluation of five methods for determining low-density lipoprotein cholesterol (LDL-C) in hemodialysis patientsen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.secondary<Go to ISI>://000174033100001-
heal.identifier.secondaryhttp://ac.els-cdn.com/S0009912001002740/1-s2.0-S0009912001002740-main.pdf?_tid=35577f0458a5409459842e241953832e&acdnat=1333110944_40e5aabe0419f6f5e75b37516c782ba1-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.publicationDate2001-
heal.abstractObjectives: Current recommendations for the management of dyslipidemia are largely based on the concentration of LDL-C. Most clinical laboratories estimate the concentration of LDL-C by the recommended routine method, the equation of Friedewald, in specimens from fasting subjects and with TG concentrations < 4.52 mmol/L. Because of the limitations of the Friedewald calculation, direct methods for an accurate quantification of LDL-C are needed. Design and Methods: In the present study we evaluated the accuracy of the following 5 different procedures for LDL-C in 98 patients on hemodialysis: the Friedewald equation, where LDL-C is calculated from HDL-C, measured either by the precipitation procedure with dextran sulfate-Mg2+ (Method 1), or by a direct HDL-C assay (Method 2), the Direct LDLTM assay (Method 3), the homogeneous N-geneous(TM) LDL assay (Method 4) and the calculated LDL-C values deriving from the ApoB based equation: 0.41TC - 0.32TG + 1.70ApoB - 0.27, (Clin Chem 1997;43:808-815) (Method 5). Results: All five LDL-C methods were found to be in good agreement with ultracentrifugation/dextran sulfate-Mg2+ precipitation with the coefficients of correlation of the assays to ranging between 0.93-0.95. However, significant differences in the mean values and biases vs. the reference method were observed. The Friedewald equation and the Direct assay were less affected by high LDfrC levels, and they presented higher sensitivity and higher negative predictive value. The N-geneous assay and the ApoB derived calculation were less affected by high triglyceride levels, and they presented higher specificity and higher positive predictive value. At the diagnostic LDL-C level of 3.37 mmol/L, both Friedewald calculations correctly classified 82/92 patients; Direct assay 86/98; N-geneous assay 88198; and ApoB derived calculation 88/98. At the diagnostic LDL-C level of 2.98 mmol/L., Friedewald calculations (Method 1 and Method 2) correctly classified 82/92 and 81/92 patients, respectively; Direct assay (LDL-3) 87/98; N-geneous assay (LDL-4) 91/98; and ApoB derived calculation (LDL-5) 91/98. Conclusions: Among hemodialysis patients, who commonly present "average" LDL-C concentrations and high TG levels, the N-geneous assay and the apoB derived calculation seem to yield more acceptable results for the estimation of LDL-C. (C) 2002 The Canadian Society of Clinical Chemists. All rights reserved.en
heal.journalNameClin Biochemen
heal.journalTypepeer reviewed-
heal.fullTextAvailabilityTRUE-
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