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https://olympias.lib.uoi.gr/jspui/handle/123456789/10385Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Yao, D. | en |
| dc.contributor.author | Vlessidis, A. G. | en |
| dc.contributor.author | Evmiridis, N. P. | en |
| dc.date.accessioned | 2015-11-24T16:56:03Z | - |
| dc.date.available | 2015-11-24T16:56:03Z | - |
| dc.identifier.issn | 0003-2670 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/10385 | - |
| dc.rights | Default Licence | - |
| dc.subject | acetylcholine | en |
| dc.subject | choline | en |
| dc.subject | chemiluminescence | en |
| dc.subject | luminol | en |
| dc.subject | enzyme immobilization | en |
| dc.subject | membrane | en |
| dc.subject | hydrogen-peroxide | en |
| dc.subject | flow-injection | en |
| dc.subject | liquid-chromatography | en |
| dc.subject | electrode | en |
| dc.subject | sensors | en |
| dc.subject | glucose | en |
| dc.subject | oxidase | en |
| dc.subject | elimination | en |
| dc.subject | biosensors | en |
| dc.title | Development of an interference-free chemiluminescence method for monitoring acetylcholine and choline based on immobilized enzymes | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.primary | Doi 10.1016/S0003-2670(02)00336-7 | - |
| heal.identifier.secondary | <Go to ISI>://000176637400007 | - |
| heal.identifier.secondary | http://ac.els-cdn.com/S0003267002003367/1-s2.0-S0003267002003367-main.pdf?_tid=925857ac-356a-11e3-8d76-00000aacb360&acdnat=1381821939_ed85e9ac473407d5abb9b868abf22e1e | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας | el |
| heal.publicationDate | 2002 | - |
| heal.abstract | Anew detection method for measurement of acetylcholine (Ach) and choline (Ch) was developed, the H2O2 that is generated in enzymatic conversion action from Ach or Ch was detected through the luminol-hexacyanoferrate based chemiluminescence (CL) reaction. A new enzyme immobilization method was developed and an interference-free membrane was prepared to achieve a higher sensor response with good selectivity and stability for detection of Ach and Ch. A novel sample preparation method was developed to extract Ach and Ch from nerve tissue efficiently, and the Ach and Ch were detected by Ach and Ch sensors with very high sensitivity and reasonable precision. The Ch sensor has a detection limit (signal/noise = 3) of 0.03 pmol (0.25 nM x 120 mul) with a linear range of 4 x 10(-8) to 1 x 10(-4) M. The Ach sensor has a detection limit of 1.2 pmol (10 nM x 120 mul) with linear range of 6 x 10(-8) to 1 x 10(-4) M. The sensitivity was improved more than 10-fold compared to the previous CL method. (C) 2002 Elsevier Science B.V. All rights reserved. | en |
| heal.publisher | Elsevier Masson | en |
| heal.journalName | Analytica Chimica Acta | en |
| heal.journalType | peer reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά). ΧΗΜ | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Yao-2002-Development of an in.pdf | 112.69 kB | Adobe PDF | View/Open Request a copy |
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