Please use this identifier to cite or link to this item:
https://olympias.lib.uoi.gr/jspui/handle/123456789/10203Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Vargas, C. | en |
| dc.contributor.author | Kallimanis, A. | en |
| dc.contributor.author | Koukkou, A. I. | en |
| dc.contributor.author | Calderon, M. I. | en |
| dc.contributor.author | Canovas, D. | en |
| dc.contributor.author | Iglesias-Guerra, F. | en |
| dc.contributor.author | Drainas, C. | en |
| dc.contributor.author | Ventosa, A. | en |
| dc.contributor.author | Nieto, J. J. | en |
| dc.date.accessioned | 2015-11-24T16:54:49Z | - |
| dc.date.available | 2015-11-24T16:54:49Z | - |
| dc.identifier.issn | 0723-2020 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/10203 | - |
| dc.rights | Default Licence | - |
| dc.subject | osmoadaptation | en |
| dc.subject | chromohalobacter salexigens | en |
| dc.subject | membrane lipids | en |
| dc.subject | ectoine | en |
| dc.subject | fatty-acid-composition | en |
| dc.subject | compatible solute ectoine | en |
| dc.subject | halomonas-elongata | en |
| dc.subject | salt concentration | en |
| dc.subject | escherichia-coli | en |
| dc.subject | pseudomonas-halosaccharolytica | en |
| dc.subject | growth temperature | en |
| dc.subject | biosynthesis | en |
| dc.subject | stress | en |
| dc.subject | tolerance | en |
| dc.title | Contribution of chemical changes in membrane lipids to the osmoadaptation of the halophilic bacterium Chromohalobacter salexigens | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.primary | DOI 10.1016/j.syapm.2005.03.014 | - |
| heal.identifier.secondary | <Go to ISI>://000231799700001 | - |
| heal.identifier.secondary | http://ac.els-cdn.com/S0723202005000834/1-s2.0-S0723202005000834-main.pdf?_tid=3b89d292-357f-11e3-96b3-00000aab0f6c&acdnat=1381830812_fb139543cba4e6e8a3bc9231f9ae7822 | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας | el |
| heal.publicationDate | 2005 | - |
| heal.abstract | The long-term response of the broad-salt growing halophile Chromohalobacter salexigens DSM 3043(T) to salt stress has been investigated with respect to adaptive changes in membrane lipid composition. This study included the wildtype and three salt-sensitive, ectoine-deficient strains: CHR62 (ectA::Tn1732, unable to grow above 0.75M NaCl), CHR63 (eetC::Tn1732, unable to grow above 1.5 M NaCl), and CHR64, which was able to grow in minimal medium M63 up to 2.5 M NaCl, but its growth was slower than the wild-type strain at salinities above 1.5 M NaCl. This mutant accumulated ectoine and hydroxyectoine as major compatible solutes, but also the ectoine precursor, N-gamma-acetyldiaminobutyric acid, and was found to be affected in the ectoine synthase gene ectC. The main phospholipids of the wild-type strain were phosphatidylethanolamine, phosphatidylglycerol (PG), and cardiolipin (CL). Major fatty acids were detected as 16:0, 18:1, and 16:1, including significant amounts of cyc-19:0, and cyc-17:0. CL and cyclopropane fatty acids (CFA) levels were elevated when the wild-type strain was grown at high salinity (2.5 M NaCl). Membranes of the most salt-sensitive trains CHR62 and CHR63, but not of the less salt-sensitive strain CHR64, contained lower levels of CL. The proportion of cyc-19:0 in CHR64 was three-fold (at 2.0 M NaCl) and 2.5-fold (at 2.5 M NaCl) lower than that of the wild type, suggesting that this mutant has a limited capacity to incorporate CFA into phospholipids at high salt. The addition of 1 mM ectoine to cultures of the wild-type strain increased the ratio PG/ CL from 1.8 to 3.3 at 0.75 M NaCl, and from 1 to 6.5 at 2.5 M NaCl, and led to a slight decrease in CFA content. Addition of 1 mM ectoine to the mutants restored the steady-state levels of CL and CFA found in the wild-type strain supplemented with ectoine. These findings suggest that exogenous ectoine might attenuate the osmostress response involving changes in membrane lipids. (c) 2005 Elsevier GmbH. All rights reserved. | en |
| heal.publisher | Elsevier | en |
| heal.journalName | Systematic and Applied Microbiology | en |
| heal.journalType | peer reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά). ΧΗΜ | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Vargas-2005-Contribution of chem.pdf | 359.51 kB | Adobe PDF | View/Open Request a copy |
This item is licensed under a Creative Commons License
