Please use this identifier to cite or link to this item:
https://olympias.lib.uoi.gr/jspui/handle/123456789/10193
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Beaufils, C. | en |
dc.contributor.author | Alexopoulos, C. | en |
dc.contributor.author | Petraki, M. P. | en |
dc.contributor.author | Tselepis, A. D. | en |
dc.contributor.author | Coudevylle, N. | en |
dc.contributor.author | Sakarellos-Daitsiotis, M. | en |
dc.contributor.author | Sakarellos, C. | en |
dc.contributor.author | Cung, M. T. | en |
dc.date.accessioned | 2015-11-24T16:54:46Z | - |
dc.date.available | 2015-11-24T16:54:46Z | - |
dc.identifier.issn | 0006-3525 | - |
dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/10193 | - |
dc.rights | Default Licence | - |
dc.subject | apolipoprotein a-i | en |
dc.subject | amphipathic alpha-helix | en |
dc.subject | atheroprotective peptide models | en |
dc.subject | hdl | en |
dc.subject | ldl | en |
dc.subject | 2d-nmr | en |
dc.subject | noesy | en |
dc.subject | molecular modeling | en |
dc.subject | cd | en |
dc.subject | fluorescence | en |
dc.subject | apolipoprotein-a-i | en |
dc.subject | magnetic-resonance spectroscopy | en |
dc.subject | low-density-lipoprotein | en |
dc.subject | solid-phase synthesis | en |
dc.subject | 3-dimensional structures | en |
dc.subject | oxidative modification | en |
dc.subject | structural-analysis | en |
dc.subject | secondary structure | en |
dc.subject | cholesterol efflux | en |
dc.subject | nmr-spectroscopy | en |
dc.title | Conformational study of new amphipathic alpha-helical peptide models of apoA-I as potential atheroprotective agents | en |
heal.type | journalArticle | - |
heal.type.en | Journal article | en |
heal.type.el | Άρθρο Περιοδικού | el |
heal.identifier.primary | Doi 10.1002/Bip.20651 | - |
heal.identifier.secondary | <Go to ISI>://000245937400004 | - |
heal.identifier.secondary | http://onlinelibrary.wiley.com/store/10.1002/bip.20651/asset/20651_ftp.pdf?v=1&t=h0e0j28c&s=9fbd1743dff12c6027f907bf0a89436d47b8c4e8 | - |
heal.language | en | - |
heal.access | campus | - |
heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας | el |
heal.publicationDate | 2007 | - |
heal.abstract | Aiming at contributing to the development of potential atheroprotective,agents, we report on the concept and design of two peptide models, which mimic the amphipathic helices of apoA-I and incorporate Met into their sequences to validate its role as oxidant scavenger. Ac-ESK(Palm)(KELSKSWSEMLKEK)-S-10-L-13(Palm)SKS-NH2 (model 1 [W-10, M-13]) and Ac-ESK(Palm)(KELSKSMSEW13)-S-10 LKEK(Palm)SKS-NH2 (model 2 [M-10 W-13]). Hydrophobic residues of both models cover about the half of the surface, while the positively and negatively charged residues constitute two separate clusters on the hydrophilic face. Palmitoyl groups were introduced into the Lys-(MH2)-H-s groups at positions 3 and 17 to contribute to the amphipathic character of the peptides and stabilize the nonpolar face of the helix. Conformational study by the combined application of 2D-NMR and molecular dynamics simulations, CD, FTIR, and fluorescence spectroscopy revealed that model I adopts helical conformation and Met is well exposed to the microenvironment. Model 2 that derives from model 1 by exchanging W-10 (model 1) with M-10 and M-13 (model 1) with W-13 also displays helical characteristics, while Met is rather shielded. Oxidation experiments indicated that model I exhibits a 2-fold more potent antioxidant activity towards LDL oxidation, compared to model 2, confirming the role of Met, when is devoid of steric hindrances, as oxidant scavenger for the protection of LDL. (c) 2006 Wiley Periodicals, Inc. | en |
heal.publisher | Wiley | en |
heal.journalName | Biopolymers | en |
heal.journalType | peer reviewed | - |
heal.fullTextAvailability | TRUE | - |
Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά). ΧΗΜ |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Beaufils-2007-Conformational study.pdf | 387.13 kB | Adobe PDF | View/Open Request a copy |
This item is licensed under a Creative Commons License