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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/13919
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dc.contributor.authorTzialla, A. A.en
dc.contributor.authorKalogeris, E.en
dc.contributor.authorGournis, D.en
dc.contributor.authorSanakis, Y.en
dc.contributor.authorStamatis, H.en
dc.date.accessioned2015-11-24T17:34:01Z-
dc.date.available2015-11-24T17:34:01Z-
dc.identifier.issn1381-1177-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/13919-
dc.rightsDefault Licence-
dc.subjectchloroperoxidaseen
dc.subjectternary systemsen
dc.subjectsurfactant free microemulsionsen
dc.subjectstabilityen
dc.subjectoxidationen
dc.subjectelectron-paramagnetic resonanceen
dc.subjectinterface-binding chloroperoxidaseen
dc.subjectdetergentless microemulsionsen
dc.subjectselective oxidationsen
dc.subjectenzymatic-reactionsen
dc.subjectthiolate ligationen
dc.subjectnonaqueous mediaen
dc.subjectionic liquiden
dc.subjectsilica-gelen
dc.subjectheme ironen
dc.titleEnhanced catalytic performance and stability of chloroperoxidase from Caldariomyces fumago in surfactant free ternary water-organic solvent systemsen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.primaryDOI 10.1016/j.molcatb.2007.10.006-
heal.identifier.secondary<Go to ISI>://000252522600004-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Μηχανικών Επιστήμης Υλικώνel
heal.publicationDate2008-
heal.abstractSurfactant free ternary systems formulated with organic solvents such as alpha-pinene or n-hexane, short chain alcohols and water, were used as new reaction media to maximize the catalytic performance of chloroperoxidase from Caldariomyces fumago (CPO) towards the oxidation of hydrophobic compounds including olefins and terpenols. The influence of the composition and the hydration state of the system, the nature of the oxidizing agent as well as substrate availability on the catalytic behavior and stability of the enzyme was investigated, using the chlorination of monochlorodimedone (MCD) and the epoxidation of styrene as model reactions. Based on residual chlorination activity measurements, which were in accordance with differential scanning calorimetry (DSC) findings, it was concluded that in low water systems formulated with alpha-pinene CPO stability is significantly increased compared to other reaction media (up to 65% residual activity after 24 h of incubation at 30 degrees C). Electron paramagnetic resonance (EPR) spectroscopy indicated that in low water content systems the ferric environment of the active site of CPO does not undergo severe modifications. Total turnover numbers (TTN) obtained for the oxidation of various olefins and terpenols in alpha-pinene-based systems, were up to 25,000 which is 2-170 times higher than previously reported values. The results indicate that such ternary systems can be efficiently employed as reaction media leading to highly enhanced stability and increase CPO potential as practical catalyst for oxidation of large amounts of hydrophobic compounds in a single-step biocatalytic process. (c) 2007 Elsevier B.V. All rights reserved.en
heal.publisherElsevieren
heal.journalNameJournal of Molecular Catalysis B-Enzymaticen
heal.journalTypepeer reviewed-
heal.fullTextAvailabilityTRUE-
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